摘要
目的探讨丝素纤维（SF）／磷酸钙骨水泥（CPC）新型复合材料修复兔桡骨骨缺损的效果。方法选取4个月龄新西兰大耳白兔48只制作兔双侧桡骨骨缺损模型，根据植入材料不同分为4组（n=12）：SF／CPC组（植入SF／CPC新型复合材料）、SF／CPC颗粒骨组（植入SF／CPC新型复合材料和微小颗粒骨）、颗粒骨组（单纯植入微小颗粒骨）和空白对照组（不植入任何材料）。术后给予相同饲养环境，并于术后4、8周时取出标本进行大体观察、x线检查、组织切片及扫描电镜观察，以及生物力学检测。结果术后x线片示空白对照组骨缺损未修复，骨缺损断端硬化，而其他3组骨缺损均不同程度修复。根据影像学检查、组织学及扫描电镜，观察显示：术后4周SF／CPC组骨小梁形成较SF／CPC颗粒骨组和颗粒骨组均细小，术后8周3组骨小梁形成均粗大、成熟。术后4周生物力学检测显示SF／CPC组最大弯曲载荷分别与SF／CPC颗粒骨组和颗粒骨组比较，差异均有统计学意义（P〈0．05），而术后8周3组间两两比较差异均无统计学意义（P〉0．05）。结论SF／CPC新型复合材料有良好的生物相容性及骨诱导性，其力学强度与富含微小颗粒骨的复合材料相似，具备一定的骨缺损修复能力。
Objective To explore the effects of silk fiber and calcium phosphate cement （SF/CPC）, a new composite material, on repairing bone defects in rabbits. Methods Models of bilateral radial bone defects were created in 48 New Zealand big-eared white rabbits which were subsequently divided into 4 even groups according to the materials used to fill the defects： group A where SF/CPC was used, group B where SF/CPC plus mieromorselized bone was used, group C where mieromorselized bone was used alone, and group D where nothing was used. The rabbits were raised in the same conditions after operation. Samples were harvested at 4 and 8 weeks postoperation for gross observation, X-ray, histopathologie and scanning electron microscopy examinations; biomeehanical tests were also conducted at 4 and 8 weeks postoperation for statis- tical analyses. Results X-ray examinations found no repair but sclerosis of bone ends in group D while different extents of bone repair were observed in the other 3 groups. According to the X-ray, histopathologieal and scanning electron microscopy examinations, trabecular bone formation in group A was thinner than in groups B and C at 4 weeks. Bulky and mature trabecular bone formation was observed in all groups at 8 weeks. Bi^mechanica^testssh^wedgr^upAhadasignificant^yweakerstrengththangr^upsBandCat4weeks （P 〈0.05）, but there were no significant differences among groups A, B and C at 8 weeks （ P 〉 0. 05）. Conclusion The new composite material SF/CPC has a certain capability of repairing bone defects, because it possesses bio- compatibility and osteoinductive ability, and similar strength of micromorselized bone composite as well.
出处
《中华创伤骨科杂志》
CAS
CSCD
北大核心
2014年第1期62-66,共5页
Chinese Journal of Orthopaedic Trauma
基金
黑龙江省卫生厅科研课题（2009-030）
哈尔滨市青年创新人才基金（2009RFQXS222）
关键词
桡骨
组织工程
修复外科手术
丝素纤维
磷酸钙骨水泥
Radius
Tissue engineering
Reconstructive surgical procedures
Silk fiber
Calcium phosphate cement
