摘要
以交联聚苯乙烯为载体的手性螯合树脂与过渡金属离子形成的络合物作配体交换色谱固定相,可拆分多种DL-氨基酸。但载体疏水性强,氮基酸在色谱过程中传质阻力大,保留时间长;硅胶键合的配体交换色谱手性相亲水性好,但柱容量小。我们通过2-羟基丙撑间隔臂将L-脯氨酸功能基联于球形酚醛树脂上,合成了手性螯合树脂(Ⅰ)。以其铜(Ⅱ)络合物作配体交换色谱固定相,对多种DL-氨基酸呈现拆分活性,且保留时间少于1h。
The microspheric phenol-formaldehyde copolymer, prepared by suspension polymerization from phenol and formaldehyde, was grafted by L-proline through 2-hydroxy propylene spacer to give a chiral chelating resin. Its copper(Ⅱ) chelate could be used as the stationary phase for the ligand-ex-change chromatography to resolve the racemates of proline, valine, isoleucine, methionine, histidine, asparagine,aspartic acid, and glutamic acid respectively.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1991年第3期421-422,共2页
Chemical Journal of Chinese Universities
关键词
酚醛树脂
手性
螯合树脂
载体
Chiral chelating resin, Ligand-exchange chromatography, Resolution of DL-amino acids
