摘要
目的 :建立登革病毒的多引物PCR检测方法 ,并对不同血清型的登革病毒进行快速分型。方法 :根据不同型别登革病毒基因组之间的保守性及型特异核苷酸序列合成相关引物 ,采用RT PCR的方法扩增登革病毒RNA ,电泳观察扩增产物的特异性和分型情况。结果与结论 :组合引物的扩增产物具有登革病毒的型别特异性 ,可对登革病毒进行快速分型 ,该方法具有敏感、特异、快速等优点。
Objective: To establish the multi primer PCR assay for detection and typing of dengue viruses. Methods: Relative primers were synthesized according to conservative and type specific sequences of nucleotides of genomes of dengue viruses. RNAs of dengue viruses were amplified by means of RT PCR. Specificity and typing of amplification products were observed by electrophoresis. Results and conclusion: Specific bands of dengue viruses were obtained and the viruses could be typed by multi primers RT PCR. The results suggest that the assay can serve as a sensitive, specific, and rapid tool for laboratory and clinical use in endemic areas of dengue fever. [
出处
《军事医学科学院院刊》
CSCD
北大核心
2000年第4期289-291,共3页
Bulletin of the Academy of Military Medical Sciences
基金
国家自然科学基金!资助项目 (39770 0 36 )
