摘要
目的 :测定新分离登革 2型病毒福建 (FJ 10 )株 5′和 3′端非编码区序列 ,分析二级结构与功能的关系。方法 :从FJ 10株感染的乳小鼠脑中提取总RNA ,利用RACE法分别扩增 5′和 3′端cDNA片段 ,并克隆至pGEM TEasy载体中 ,然后分别测定插入片段的核苷酸序列 ,并用RNA绘图软件预测 5′和 3′端非编码区的二级结构。结果和结论 :FJ 10株 5′和 3′端非编码区长度分别为 96和 4 54个核苷酸 ,并形成复杂的茎环结构 ,可能与病毒的复制和毒力有关。
Objective:To determine 5′ and 3′ noncoding region sequences of a new dengue 2 virus FJ 10 strain and to analyze the relationship between the secondary structure and the functions. Methods:Total RNA was extracted from a suckling mouse brain infected by the dengue 2 virus FJ 10 strain. Following the reverse transcription, the cDNAs of both 5′ and 3′ termini were amplified using RACE method, respectively. The cDNAs were cloned into the pGEM T Easy vector and sequenced, and the secondary structures of the 5′ and 3′ termini were predicted with the RNAdraw software. Results and Conclusions: The 5′ and 3′ noncoding regions comprise 96 and 454 nucleotides, respectively , and are predicted to fold into complicated stem loop structures which are possibly correlated with viral replication and virulence.
出处
《军事医学科学院院刊》
CSCD
北大核心
2000年第4期259-262,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
登革2型病毒
碱基序列
序列分析
二级结构
PCR
dengue 2 virus
base sequence
sequence analysis
secondary structure
polymerase chain reaction
