摘要
目的 :研究中药开心散对肾缺血再灌注损伤的保护作用。方法 :复制大鼠肾缺血再灌注损伤模型 ,用开心散治疗 ,观察肾组织病理变化 ,并对肾组织LDH用显微镜 计算机真彩色图像分析系统分析 ;测定血清和肾组织SOD、MDA、NO含量。结果 :肾缺血 1h灌注 15min后 ,肾组织出现明显病理改变 ,肾小管LDH活性增加 ;血清和肾组织SOD、NO活性下降 ,MDA含量升高。应用开心散治疗后血清和肾组织SOD、NO活性升高 ,MDA含量下降 ,肾组织病理变化有所改善。结论 :开心散具有减轻脂质过氧化反应、清除自由基、保护血管内皮细胞等作用 ,这可能是开心散减轻肾缺血再灌注损伤作用机制之一。
Objective:To study the protective actionof Kaixin powder on the kidney in the cases of ischemia-reperfusion injury.Method:The models of ischemia-reperfusion injury were constructed in rats.The pathological changes of the tissues in the kidney were observed and shades of lactic acid dehydrogenase (LDH)in the kidney tissue were analyzed with computer.The content of superoxide dismutase(SOD),malondialdihyde(MDA) and nitric oxide(NO) in the serum and kidney tissues were measured.Result:After ischemia of the kidney tissues was kept for 1 hour and reperfusion 15 minutes,significant pathological changes in the kidney tissues was kept for 1 hour and LDH in the renal tubulars were increased.The activity of SOD and NO in the serum and kidney tissue were decreased and the content of MDA were increased.After Kaixin powder was administered,the activity of SOD and NO were increased,the content of MDA were lowered and the pathological changes of the kidney tissues were improved.Conclusion:Kaixin powder can alleviate lipid peroxidation,get rid of free radicals and protect the endothelial cell of blood vessels.This may be one of the mechanisms that improve ischemia-reperfusion injury of the kidney.
出处
《安徽中医学院学报》
CAS
2000年第6期54-56,共3页
Journal of Anhui Traditional Chinese Medical College
基金
:南京中医药大学自然科学基金
关键词
肾缺血
再灌注损伤
开心散
中医药疗法
保护作用
Kidney ischemia-reperfusion injury
Ischemic kidney injury Free radical
Kaixin powder
