摘要
通过分子动力学模拟的方法,模拟了1-棕榈酰基-2-油酰基磷脂酰乙醇胺(1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine,POPE)生物膜在添加胚胎发育晚期丰富蛋白(Late embryogenesis abundant proteins,LEA蛋白)特征重复片段(LEA-motif)前后两种体系在低温下的干燥过程,对比分析了干燥过程中两种体系在POPE生物膜结构、扩散系数、侧链有序性及分子间氢键数目的变化,从微观角度揭示了POPE生物膜因干燥失水导致的结构变化以及添加LEA-motif之后LEA-motif与POPE生物膜的相互作用.结果表明,LEAmotif在脱水干燥过程中能够有效地稳定膜的结构,从而保护生物材料的生物活性.
Late embryogenesis abundant（LEA） protein is a kind of low molecular weight and high hydrophilic protein and the expressions of it are correlated with desiccation tolerance. Combined with their multitudinous biological functions, such as stabilizing membranes and proteins, scavenging free radicals and so on, LEA would be good and potential preotectant for biomaterials. However, the protective mechanism of LEA protein for membrane is not clear and definite, which results in the absence of abrupt development for the actual appli- cations. Therefore, the drying process of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine （POPE） membrane with or without LEA-motif protectant at low temperature was thoroughly researched by molecular dy- namics simulation. The results such as the 3D structure of POPE membrane, the lateral diffusion coefficient of POPE lipids, the order parameter of hydrophobic chains and the H-bonding interactions were analysized and compared between the two systems detailedly. The damage of POPE membrane and the bioactive protection of LEA-motif to the POPE membrane during desiccation were revealed from the microscopic view. The results in- dicate that LEA-motif can stabilize the structure of POPE membrane and then maintain the biological activity of biomaterials.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2014年第1期161-167,共7页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:51076108)
上海市国际合作项目(批准号:12430702000)
上海市自然科学基金(批准号:12ZR1420400)
上海市教委科研创新项目(批准号:14YZ092)
上海市联盟计划项目资助~~
关键词
干燥
POPE生物膜
LEA蛋白特征重复片段(LEA—motif)
分子动力学模拟
Desiccation
1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine （ POPE ） membrane
Lateembryogenesis abundant（LEA）-motif
Molecular dynamics simulation
