摘要
目的为了建立以尿液为标本的检测沙眼衣原体的聚合酶链反应（ＰＣＲ）－微孔板杂交法。方法以１００例性病门诊就诊者的尿液和尿道（男性）或宫颈（女性）标本，分别作沙眼衣原体ＰＣＲ－微孔板杂交法和细胞培养。两者结果不一致时用第二种不同引物作ＰＣＲ。结果与“扩大金标准”比较，尿液ＰＣＲ－微孔板杂交法的敏感性、特异性、阳性预期值和阴性预期值分别为８０． ０％、９７． ３％。９０．９％和９３． ６％；细胞培养的敏感性、特异性、阳性预期值和阴性预期值分别为８４． ０％、１００． ０％、１００．０％和９４．９％。结论尿液ＰＣＲ－微孔板杂交法检测沙眼衣原体感染的技术性能与细胞培养相当，但有取材方便、快速的特点，将其用于性病门诊就诊者的诊断是可行的。
Objective To set up an in house method for detection of genital Chlamydia trachomatis infection by polymerase chain reaction (PCR) - microplate hybridization in urine. Methods The urine and urethral or cervical specimens were taken from 100 sexually transmitted disease clinic attendees. The results of cell culture and PCR were compared. The discrepant results were confirmed by the PCR using a second set of primes. Results In comparison with expanded gold standard, the sensitivity,specifici- ty, positive predictive value and negative predictive value were 80. 0 %, 97. 3 %, 90. 9 % and 93. 6 % for PCR, and 84 %, 100 %, 100% and 94. 9% for cell culture, respectivaly, Conclusions PCR in urine specimens provides a sensitive, specific, noninvasive method for detection of genital chlamydia trachomatis infection,and is feasible for application in sexually transmitted disease clin- ics.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2000年第6期369-371,共3页
The Chinese Journal of Dermatovenereology
