摘要
目的 :探讨精氨酸增强 TPN对急性胰腺炎 (AP)大鼠肠粘膜屏障的影响。 方法 :雄性 SD大鼠 6 4只 ,随机分成 :1对照组 (n=16 ) ;2 AP组 (n=16 ) ;3AP+TPN组 (TPNs组 n=16 ) ;4AP+TPN+精氨酸组 (TPNa组 n=16 )。分别于建立急性胰腺炎模型后第 1及第 5天剖杀每组 8只大鼠取材 ,检测肠粘膜一氧化氮 (NO)、丙二醛、蛋白质含量、肠系膜淋巴结及门静脉血细菌移位率。 结果 :与对照组比较 ,术后 1天及 5天时 ,AP组肠粘膜丙二醛含量及肠系膜淋巴结细菌移位率显著升高 ;5天时蛋白质含量显著降低 ,而门静脉血细菌移位率明显升高。与 TP-Ns组比较 ,TPNa组 1天时肠粘膜 NO含量即明显升高 ;肠系膜淋巴结细菌移位率显著降低 ;5天时肠粘膜 NO与蛋白质含量均显著升高 ;丙二醛含量显著降低。 结论 :急性胰腺炎可引起肠粘膜屏障损害 ;精氨酸增强 TPN具有保护肠粘膜屏障的作用。
Objectives:To investigate the effect of arginine enriched TPN on gut barrier of rats with acute pancreatitis. Methods:64 SD rats were divided into four groups at random:1.Control group(sham operated, n =16);2.AP group( n =16);3.AP+TPN group(TPNs group, n =16);4.AP+TPN+Arginine group (TPNa group, n =16).Every 8 rats in each group were sacrificed on day 1 and day 5 after inducing acute pancreatitis respectively.The level of nitric oxide(NO),malondialdehyde,protein in intestinal mucosa and bacterial translocation rates were measured. Results:Compared with control group on day 1 and day 5 after induction of acute pancreatitis.The level of malondialdehyde in intestinal mucosa and bacterial translocation rates of mesenteric lymphaden of AP group increased significantly( P <0.01, P <0.05);The level of protein in intestinal mucosa decreased and bacterial translocation rates in portal vein increased significantly on day 5( P <0.01).Compared with TPNs group,the level of NO in intestinal mucosa increased and bacterial translocation rates of mesenteric lymphaden of TPNa group decreased significantly on day 1( P <0.05).On day 5,the level of NO and protein in intestinal mucosa increased significantly( P <0.05, P <0.01),and malondialdehyde decreased significantly( P <0.01). Conclusion:Acute pancreatitis may cause the damage of gut barrier;Arginine enriched TPN has protective effect on gut barrier of rats with acute pancreatitis.
出处
《肠外与肠内营养》
CAS
2000年第4期196-199,共4页
Parenteral & Enteral Nutrition
关键词
急性胰腺炎
精氨酸
全肠外营养
肠粘膜屏障
Acute pancreatitis
Arginine
Total parenteral nutrition
Nitric oxide
Gut barrier
