摘要
首次利用一株安全菌株解淀粉芽胞杆菌发酵生物柴油副产物粗甘油生产2,3-丁二醇。溶氧和pH是影响微生物法生产2,3-丁二醇的最主要因素。结果表明,发酵过程中不控制pH更有利于2,3-丁二醇合成;采用三阶段控制搅拌转速策略,2,3-丁二醇产量最大值达到38.1 g/L,生产强度达到1.06 g/(L·h),与恒定转速获得的最好结果相比,分别提高了14.8%和63.1%。采用脉冲流加发酵时,2,3-丁二醇产量达到71.2 g/L,2,3-丁二醇生产强度达到0.99 g/(L·h),这是目前报道的利用粗甘油合成2,3-丁二醇的最高产量。
Bacillus amyloliquefaciens B10-127 was used to produce 2,3-butanediol （2,3-BD） from residual glycerol obtained from biodiesel synthesis. Important variables for 2,3-BD fermentation, pH and dissolved oxygen, were studied. When pH was maintained constant, the yield of 2,3-BD was inhibited. The highest 2,3-BD yields were achieved by fermentation without any pH control with an optimized initial pH 6.5. Batch fermentative production of 2,3-BD by B. amyloliquefaciens was investigated using various oxygen supply methods by changing agitation speed. Based on the analysis of three kinetic parameters including specific cell growth rate （μ）, specific glucose consumption rate （qs） and specific 2,3-BD formation rate （qp）, a three-stage agitation speed control strategy was proposed, aimed at achieving high concentration, high yield and high productivity of 2,3-BD. Maximum concentration of 2,3-BD rea,zhed 38.1 g/L, with the productivity of 1.06 g/（L·h）, which were 14.8% and 63.1% over the best results from constant agitation speeds. In a pulse fed-batch fermentation, 2,3-BD concentration and productivity were significantly improved to 71.2 g/L and 0.99 g/（L-h）, respectively. To our knowledge, these results were the highest for 2,3-BD production from biodiesel-derived glycerol.
出处
《生物工程学报》
CAS
CSCD
北大核心
2013年第12期1860-1864,共5页
Chinese Journal of Biotechnology
基金
教育部新世纪优秀人才计划(No.NCET-10-0459)
国家重点基础研究发展计划(973计划)(No.2012CB725202)
国家高技术研究发展计划(863计划)(No.2011AA02A211)
国家自然科学基金(Nos.21276110
30970056)
中央高校基本科研业务费专项资金(No.JUSRP51306A)
高等学校博士学科点专项科研基金(No.20110093120001)
江苏高校优势学科建设工程资助项目资助~~
关键词
解淀粉芽胞杆菌
粗甘油
PH
三阶段控制转速
流加发酵
2
3-丁二醇
Bacillus amyloliquefaciens, biodiesel-derived glycerol, pH, three-stage control strategy, fed-batch, 2,3-butanediol
