摘要
目的： 建立气相色谱法测定人参皂苷Rg1中有机残留量的方法。方法： 采用气相色谱法，FID检测器， DB-624 （30 m×0.32 mm，0.25 μm），载气为氮气，柱温为恒温50℃，进样口温度200℃，检测器温度200℃，以水为溶剂配制对照溶液及供试品溶液。结果： 在所考察的浓度范围内具有良好的线性，乙醇的检测限为1.0 μg·L-1，精密度RSD〈1.0%。平均回收率为90%~105% 结论： 经方法学验证，该检测方法简便灵敏，结果准确可靠，适用于人参皂苷Rg1原料药中有机溶剂残留量的检测。
Objective： To establish a method for the determination of residual solvents in Rg1 by GC. Method： The detector was FID.The analytical column was DB-624（0.32 mm×30 m,0.25 μm）.The injector temperature was 200℃,and the detector temperature was 200℃.The column temperature was 50℃.Nitrogen as the carrier gas and the water as solvent media. Result： It had a good linearity in the experimental concentration.The detection limit of ethanol was 1.0 ng·mL-1.The RSD of precision was all less than 1.0%.The average recovery was 90%-105%. Conclusion： This method was simple,sensitive. accurate and can be used for the detection of f residual solvents in Rg1.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第24期118-120,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技重大专项"重大新药创制"项目(2009ZX09301-005)
