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SD大鼠多余毛细胞的扫描电镜观察

THE SCANNING ELECTRON MICROSCOPIC STUDY OF SUPERNUMERY HAIR CELLS
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摘要 观察SD大鼠耳蜗多余外毛细胞分布、形态,并探讨其发育规律。方法:大鼠4只,3周龄。取出耳蜗后用2.5%戊二醛固定1小时以上,去除骨壳、前庭膜、血管纹,制作扫描电镜标本,S-520型电镜扫描观察毛细胞形态。结果:耳蜗外毛细胞排列成规则的3排。4只大鼠中有2只在耳蜗某些部位可见多达5排以上外毛细胞,多余外毛细胞部分排列紊乱,越近底端越明显。结论:SD大鼠可出现多余外毛细胞,这种多余毛细胞排列不规则。 To investigate the supernumerary hair cells of SD rat. Mathods:The cochlea were obtainedfrom four 3-week SD rats. The apex was punctured, and was then perfused through the oval window with glutaraldehyde.Specimens were post-fixed with 1% osmium-tetroxide for 30 min, then dehydraed, point-dried and sputter-coated withgold-palladium. The specimens were studied by scanning electron microscope. Results: Supernumerary outer hair cellsranged up to 5 rows were observed. Most of them rowd irregularly. Conclusions: These studies provide evidence for thepresence of supernumerary outer hair cells in the rat cochlea.
出处 《泸州医学院学报》 1999年第4期291-292,共2页 Journal of Luzhou Medical College
关键词 毛细胞 扫描电镜 SD大鼠 耳蜗 Scanning electron microscope Hair cell Rat
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