摘要
[摘要]为建立云南省龙陵县紫皮石斛的地方质量标准，采用硫酸-苯酚法测定紫皮石斛中多糖的含量；采用高效液相色谱法测定药材中甘露糖的含量，并根据2010年版《中国药典》相关附录测定浸出物。结果表明紫皮石斛的显微鉴别特征性强；薄层色谱分离度好，斑点清晰；紫皮石斛含多糖质量分数为35．7％-52．1％（平均42．7％），甘露糖为27．8％～46．1％（平均35．8％），浸出物为4．5％－10．6％（平均7．38％）。所建方法操作简单，准确可靠，重复性好，制定的标准限度合理，可用于评价云南省龙陵县紫皮石斛的质量。
To establish the local quality standard for Dendrobii devoniani caulis from Longling, Yunnan, the pharmacognostic characteristics microscopic characteristics and TLC identification were developed. Sulfuric acid-phenol method was used to determine the content of polysaccharide. An HPLC method was adopted to determine the content of mannose, and extractives were determined ac- cording to the procedures recorded in the Appendix of Chinese Pharmacopoeia（2010）. The results showed a strong characteristics mi- croscopic of Dendrobii devoniani caulis, and its TLC identification had a good resolution with clear spots ; the content of polysaccharide is 35.7% -52. 1% （ average 42. 7% ）, mannose 27.8% -46. 1% （ average 35.8% ）, and extract 4. 5% -10. 6% （ average 7.38% ）. The method is simple, accurate and reliable, with good reproducibility. The established standard is acceptable for quality evaluation of Den- drobii devoniani caulis from Longling, Yunnan.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第23期4113-4118,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金优秀青年基金项目(81222053)
