摘要
丹参是世界公认的治疗心脑血管病的首选药物，栽培丹参枯萎病发生严重，严重影响栽培丹参产量和质量。针对上述问题，采用形态学和病原学实验手段对从发生枯萎病丹参植株茎和根中分离得到的病原菌进行鉴定，同时根据ITS序列采用PCR的方法进行进一步确定。结果表明丹参枯萎病多发生7—8月高温、多雨季节，栽培一年丹参枯萎病的发生率为10％左右，而栽培三年或者三茬丹参枯萎病的发生率达到了60％～70％。枯萎病会引起丹参根腐，因此生产上易误认为根腐病。形态学鉴定结果表明该病原菌为尖孢镰刀菌，ITS序列也表明其与黄瓜枯萎病菌的ITS序列同源性为99％。因此，作者认为引起丹参枯萎病的病原菌为尖孢镰刀菌。
Salvia miltiorrhiza is a highly valued traditional chinese medicine for the treatment of atherosclerosis-related disor- ders in china, such as cardiovascular and cerebrovascular diseases in China. The wilt disease is serious in the culture of S. miltiorrhi- za. Wilt disease cause biomass of plant shoots and roots is lessened, active components are decreased. To solve these problems,we re- search the pathogen causing wilt disease of S. miltiorrhiza. The suspected pathogen is identified by morphology and etiological test. The identification was further confirmed by alignment the sequences of internal transcribed spacer（ITS） amplified by PCR. Our result show the wilt disease of S. miltiorrhiza mostly occurred in July and August, which is hot and wetter. The wilt disease rate of S. miltiorrhiza continuous cropping for one year in S. miltiorrhiz stubble is 10% , but the wilt disease rate of S. miltiorrhiza continuous cropping for three years in S. miltiorrhiz stubble is 60%-70%. The root rot of S. miltiorrhiz caused by the wilt disease, so the wilt disease was mis- taken for the rot root in production. Morphological characteristics show the pathogen is Fusarium oxysporum. The sequence of ITS wes determined and found by BLAST shared 99% identity to that of F. oxysporum f. sp. cucumerinum. So it comes to the conclusion that the causing agent of wilt disease on S. miltiorrhiza belongs to F. oxysporum.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第23期4040-4043,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金重点项目(81130070)
国家科技基础性专项(SB2007FY020)
国家中医药管理局行业科研专项(201107009
201207002)
国家科技支撑计划项目(2012BAI29B02)
关键词
丹参
枯萎病
病原菌鉴定
尖孢镰刀菌
Salvia miltiorrhiza
wilt disease
pathogen identification
Fusarium oxysporum
