摘要
目的:研究健脾渗湿方对高尿酸血症大鼠模型尿酸盐转运蛋白OAT3表达的影响,揭示其治疗高尿酸血症的作用机制。方法:40只大鼠随机分为正常对照组、模型对照组、苯溴马隆组和健脾渗湿方组,每组10只。采用酵母饲料加腺嘌呤诱导高尿酸血症大鼠模型,灌胃给药14 d后,检测高尿酸血症大鼠肾脏病理损伤,免疫组化法检测尿酸盐转运蛋白OAT3的表达情况。结果:健脾渗湿方组高尿酸血症模型大鼠肾脏的损伤程度显著改善,且对尿酸盐转运蛋白OAT3具有显著的上调作用。结论:上调尿酸盐转运蛋白OAT3,可能是健脾渗湿法治疗高尿酸血症的作用机制之一。
Objective：To study the effects of jianpi shenshi formula on the expression of urate transporters OAT3 in hyperuricemia model rats to reveal its molecular mechanism in the treatment of hyperuricemia.Methods：40 rats were randomly divided into the normal control group,the model control group,the benzbromarone positive group and the Jianpi Shenshi Formula group.The hyperuricemia rat models were induced by yeast feed and adenine and given intragastric administration for 14 days.HE staining method was used to detect the renal pathological damage of hyperuricemia rats and immunohistochemistry method was used to detect the expression of urate transporter OAT3.Results：Jianpi shenshi formula could not only improve the renal pathological damage of hyperuricemia rats but also up-regulate the urate transporter OAT3.Conclusion：The up-regulation of urate transporter OAT3 might be one of the mechanisms of jianpi shenshi formula in the treatment of hyperuricemia.
出处
《风湿病与关节炎》
2013年第12期22-25,共4页
Rheumatism and Arthritis
基金
国家自然科学基金项目(30960476)
