摘要
微管相关肿瘤抑制基因1(MTUS1)是一个新的肿瘤抑制基因,编码血管紧张素II AT受体结合蛋白(ATIPs).MTUS1基因表达下调可促进肿瘤细胞增殖,但MTUS1基因表达在结直肠癌转移中的作用尚不知晓.本文以接受根治性手术、经病理检查证实的50例结直肠癌患者新鲜癌组织、癌旁组织和远癌组织为材料,探索MTUS1基因表达与结直肠癌淋巴结转移的关系.荧光定量PCR及蛋白质免疫印迹揭示,有淋巴结转移的结直肠癌患者的癌及癌旁组织MTUS1基因表达的mRNA及蛋白质水平明显低于无淋巴结转移的患者.MTUS1蛋白质在淋巴结转移的结直肠癌患者的癌及癌旁组织表达下调,并进一步得到免疫组织化学的验证.实验结果提示,MTUS1的表达量与肿瘤的侵润深度有明显的负相关性.结合临床资料,推断抑癌基因MTUS1在肿瘤的发生、发展及淋巴转移中发挥重要作用.
Microtubule associated tumor suppressor 1 （MTUS1） as a new tumor suppressor gene codes for angiotensin II AT2 receptor interacting protein （ATIPs）. Although the down-regulation of MTUS1 promotes tumor cell proliferation, the affection of the expression of MTUS1 in colorectal cancer metastasis was unknown. We collected the 50 cases of CRC patients who received radical resection confirmed by pathology at three positions （cancer tissues, paracancer tissues and normal distal tissues） for studying the correlation between the expression of MTUS1 and the lymph node metastasis in colorectal cancer. With fluorescence quantitative PCR （qRT-PCR）, Western blot and immunocytochemistry staining, we found that the expression level of MTUS1 mRNA and protein in carcinoma tissues and adjacent noncancerous tissues was significantly lower in the cases with the lymph node metastatic CRC patients than those of the patients without lymph node metastasis （P 〈 0.05）. Combining with clinical data, our study indicated that the tumor suppressor gene MTUS1 might play an important role in tumor lymph metastasis.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2013年第12期1153-1158,共6页
Chinese Journal of Biochemistry and Molecular Biology
基金
重庆市科技攻关项目(No.cstc2012gg-yyjs0644)
吴阶平基金项目(No.320.6750.12685)~~
关键词
结直肠癌
微管相关肿瘤抑制基因1
肿瘤抑制
转移
colorectal cancer
microtubule associated tumor suppressor 1 gene （MTUS1）
tumor suppressor
metastasis
