摘要
目的 :检测精液中是否存在丙型肝炎病毒 (HepatitisCviruse,HCV) ,探讨HCV性传播途径的可能性。 方法 :用双抗体夹心ELISA法检测 147例精浆HCV抗原 ;HCV抗原阳性者检测其夫妻双方血清中抗HCV IgM和抗HCV IgG ;同时采用 90 %和 45 % 2层Percoll梯度离心 ,分离HCV抗原阳性病人的精子 ,用RT PCR分别检测其精浆(组分 1)、90 %Percoll层精子 (组分 2 )、45 %Percoll层精子 (组分 3)及最上层圆形细胞 (组分 4)中的HCV RNA。 结果 :147例精浆中HCV抗原阳性的 4例 (2 7% ) ;4例HCV抗原阳性病人夫妻双方血清抗HCV抗体均为阴性 ;4例HCV抗原阳性病人有 2例精浆中检测出HCV RNA ,而Percoll分离后 3个组分中HCV RNA均为阴性。 结论 :①HCV感染存在性传播途径 ;②人工授精中供精者HCV的检测必不可少 ;③采用Percoll梯度离心分离精子 ,可大大降低感染HCV的风险。
Objectives:[WT5”BZ] To detect hepatitis C virus in semen and to identify its risk of sexual transmission [WT5”HX] Methods:[WT5”BZ] 147 samples of seminal plasma from infertile patients were screened for the HCV core antigen C33 by a sandwich ELISA Antibodies(IgM and IgG) HCV in serum obtained from positive patients and their wives were examined by the ELISA Meanwhile, the HCV antigen postitive semen were collected The seminal fluid and cell pellet were separated after centrifugation The cell pellet was then subjected to centrifugation through a two layer(90% and 45%) discontinuous Percoll gradient HCV RNA was tested using RT PCR assay in the seminal fluid(fraction 1),spermatozoa in 90% Percoll(fraction 2), spermatozoa in 45% Percoll(fraction 3) and round cells on the 45% Percoll(fraction 4) [WT5”HX]Results:[WT5”BZ] HCV antigens were found positive in 4 cases(2 7%), while HCV antibodies were negative in serum of these 4 cases and their wives HCV RNA was detected in 2 of the seminal plasma samples from these 4 patients, but not found in the other 2 and all of the fractions after Percoll centrifugation(fraction 2,3,4) [WT5”HX]Conclusions:[WT5”BZ] HCV can be detected in semen of infertile men, indicating that sexual transmission of HCV exists actually Therefore, the HCV(in serum or semen) of donor must be screened before artificial insemination In addition,it can greatly reduce the risk of infecting HCV using spermatozoa after Percoll selection in assisted reproduction techniques
出处
《中华男科学杂志》
CAS
CSCD
2000年第4期241-243,共3页
National Journal of Andrology
