摘要
研究从斑马体内分离的蛔虫的核糖体DNA内转录间隔区(ITS)及5.8S rDNA序列的遗传变异情况,并用ITS序列重构斑马蛔虫与其它蛔虫的种群遗传关系。利用聚合酶链反应(PCR)扩增斑马蛔虫rDNA的ITS-1、5.8S及ITS-2片段,将PCR扩增出的片段纯化后克隆至pGEM-T Easy载体,重组质粒通过菌落PCR鉴定后,对阳性菌落进行序列测定并进行序列分析。结果显示所获得的斑马蛔虫ITS及5.8S rDNA序列总长为892bp,包含部分的18S、28S及全部的ITS-1、5.8S及ITS-2序列。证实从斑马体内分离的蛔虫为马副蛔虫,从而为斑马蛔虫的分类鉴定以及进一步的分子流行病学调查奠定了基础。
The objective of the study was to examine sequence variation in the internal transcribed spacer （ITS） and 5.8S rDNA ofParascaris equorum from Equus burchelli in Hunan province, and to reconstruct their phylogenetic relationship using ITS sequences. The ITS sequences were amplified from each Parascaris equorum sample and the amplicons were cloned into pGEM-T Easy vector. The inserts were successfully sequenced, and the length of ITS in all samples was 892bp in size containing part of 18S and 28S and complete ITS-I, 5.8S and ITS-2 sequences, suggesting that the ascarides from Equus burchelli was Parascaris equorum. The study lay down the foundation for further study on molecular epidemiology and diagnostics ofParascaris equorum from Equus burchelli.
出处
《中国动物检疫》
CAS
2013年第12期62-65,共4页
China Animal Health Inspection
