摘要
目的 了解北京地区病毒性心肌炎的病毒感染状况。方法 用IgM抗体捕捉ELISA(MacELISA) ,检测 193例临床诊断为病毒性心肌炎患者血清中的CoxB 1～ 6型病毒特异性IgM抗体。结果 CoxB病毒IgM抗体阳性检出率为6 9.9% (135 193) ,其中阳性检出率最高为B3型 ,占40 % (5 4例 ) ;其余依次为B2型 ,占 2 9.6 % (4 0例 ) ;B4型占 18.5 % (2 5例 ) ;B1型占 7.4% (10例 ) ;B5型占 3.0 % (4例 ) ;B6型占 1.5 % (2例 )。而 181例同年龄组非心肌炎患者IgM抗体阳性检出率为2 0 .4% (37 193)。病毒性心肌炎患者血清中的CoxBIgM抗体阳性检出率明显高于非心肌炎患者 ,两者差异有显著性 ,U =9.5 6 ,P <0 .0 1。
Objective To detect Coxsackie B virus type 1 6 IgM antibodies from 193 clinically diagnosed myocarditis cases. Methods The capture IgM ELISA (Mac ELISA) test wass used. Results The total positive rate of Coxsackie virus B 1 6 IgM antibody in myocarditis patients was 69.6 % (135 cases), among which Cox B3 type was as high as 40% (54 cases) of the total positive patients. The others were 29.6 % (40 cases), 18.5 % (25 cases), 7.4 %(10 cases), 3.0 % (4 cases), 1.5 %(2 cases) of Cox B2,Cox B4,Cox B1,Cox B5 and Cox B6 respectively. Meanwhile only 20.4% (37 cases) Cox B IgM positive cases could be detected in 181 non myocarditis patients of same ages. The results showed that the positive rate of Cox B virus IgM antibody in clinically diagnosed myocarditis was significantly higher than that in control group ( P < 0.01 ). Conclusion The Cox B viruses may be the main agents causing viral myocarditis in Beijing cases. Mac ELISA is an easy, rapid,specific and sensitive method to detect Cox B virus IgM antibody for early and rapid diagnosis of myocarditis.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2000年第4期370-372,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
柯萨奇病毒B组
心肌炎
IGM
Coxsackie B viruses
Myocarditis
Enzyme linked immunosorbent assay
IgM
