摘要
目的 研究人类免疫缺陷病毒 1型 (HIV 1)感染无进展与进展者HIVDNA存在状态的特点。方法 选用位于HIV 1DNA链两端的LTR(U5 )、LTR(R)等其他部位的不同引物 ,应用国际上新颖的长片段聚合酶链反应 (LD PCR)及逆转录PCR(RT PCR) ,普通PCR(Taq PCR)、免疫转印、分子杂交等技术 ,对HIV 1感染者血清HIVRNA、外周血单个核细胞 (PBMC)HIVDNA进行检测。结果 9.1kb为全长HIVDNA基因片段 ,12例进展者全部存在全长HIV基因片段 ,其中 ,9例存在不完整缺损的HIVDNA ;在 18例无进展者中 ,5例既存在完整又存在不完整的HIVDNA ,其余13例只存在不完整、缺损的HIVDNA。
Objective This study was designed to investigate the different status of provirus HIV DNA in non progressor and progressor of HIV 1 infected persons.Methods HIV RNA, HIV DNA were detected with long distance PCR (LD PCR),reverse transcription PCR (RT PCR),Southern blot and molecular hybridization from plasma and peripheral blood mononuclear cells (PBMCs), using primers locating between long terminal repeats LTR(U5) LTR(R) and so on. Results 9.1 kb band is full length HIV DNA genome which was presentin all templates of 12 progressors. Deleted HIV DNA was observed in 9 of 12 progressors. Both the full length and defective. provirus HIV DNA was detected in 5 of the 18 non progressors. Defective HIV DNA was only detected in the other non progressors. Conclusion Clinical stages are closely related with both the full length and the defective HIV DNA.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2000年第4期333-337,共5页
Chinese Journal of Experimental and Clinical Virology
