摘要
目的研究丝裂霉素C壳聚糖纳米粒(MMC-CS-NPs)对H_(22)荷瘤小鼠的肿瘤抑制作用。方法采用小鼠H_(22)肝癌细胞实体瘤模型进行体内抑瘤实验,观察荷瘤动物的生长情况,并测定肿瘤生长抑制率;肿瘤组织病理学切片行HE染色以进行形态学观察。结果 MMC-CS-NPs对小鼠的H_(22)实体瘤生长具有明显的抑制作用,低、中、高剂量组的抑瘤率分别为52.23%、66.93%、80.91%,与对照组相比有统计学意义(P<0.05)。病理组织学观察显示,各剂量组对肿瘤组织都有不同程度的破坏,肿瘤细胞大量变性坏死。结论 MMC-CS-NPs具有较强的体内抗肿瘤作用,与丝裂霉素针剂相比其抑瘤率更高,且与剂量有关,并对肿瘤生长的抑制更为持久。
AIM To investigate the antitumor effect on H22 tumor-bearing mice treated with mitomycin C chitosan nanoparticles（MMC-CS-NPs）. METHODS Hepatocelhdar carcinoma cell H22 was subcutaneously injected into mice and MMC-CS-NPs were administered to the tumor-bearing mice. The growth of tumor-bearing mice was observed, and the in- hibition rate of tumor growth was calculated. The tumor tissue samples were taken and examined to assess the inhibitory effects of MMC-CS-NPs on tumor growth in the mice. RESULTS Marked inhibitory effect of MMC-CS-NPs on the trans- planted hepatocellular carcinoma H22 was observed in the tumor-bearing mice. The inhibition rates were 52.23 %, 66.93 % and 80.91% in the groups treated with low, medium and high dosage of MMC-CS-NPs, respectively （ P 〈 0.05 vs control group）. Histopathological examination revealed widespread necrosis with massive accumulation of infiltrating lymphocytes and plasmacytes in the tumors. CONCLUSION MMC-CS-NPs has marked inhibitory effects on tumor gro^h in vivo. The inhibitory effects of MMC-CS-NPs are obvious and dose-dependent and higher than those of mitomycin injec- tion.
出处
《中国临床药学杂志》
CAS
2013年第6期334-337,共4页
Chinese Journal of Clinical Pharmacy
基金
厦门市科学技术计划项目资助(编号3502Z20114007)
关键词
丝裂霉素C壳聚糖纳米粒
抑瘤作用
H22肝癌细胞
mitomycin C chitosan nanoparticle
antitumor effect
hepatocellular carcinoma cell H22
