摘要
目的探讨异氟醚预处理在对大鼠皮层神经元缺氧损伤中的保护作用及其可能机制。方法建立原代培养的大鼠皮层神经元缺氧无糖损伤(OGD)模型,采用MTT法观察异氟醚对神经元OGD损伤的保护作用,Western blot检测磷酸化ERK(pERK)和低氧诱导因子(HIF)-1α蛋白表达。结果异氟醚预处理显著增加OGD损伤神经元中HIF-1α的蛋白含量,ERK抑制剂PD98059可以部分阻断这种作用。结论异氟醚预处理对于大鼠原代皮层神经元OGD损伤具有明显的保护效果。其机制可能与通过增加pERK表达,进而调节HIF-1α的表达有关。
Objective To study the protection and mechanism of isoflurane preconditioning against oxygen-glucose deprivation（OGD） neuronal injury. Methods Cortical neurons from rats at embryonic day 16 to 17 were cultured. With simuation of a cerebral ischemia in vitro, OGD was performed in the pretreated cells. Neuron injury was assessed by MTT. Protein expressionof pERK and HIF-1α was determined by Western blot. Results Isoflurane preconditioning protected dose-dependently the cortical neuron from OGD injury. The hypoxia inducible factor （HIF-1α） in the OGD injured neurons was increased by isoflurane preconditioning, which was partially abolished by ERK inhibitor PD98059. Conclusions Isoflurane preconditioning protects the cortical neuron from OGD injury, possibly through activating HIF-1α mediated by pERK.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2013年第6期702-705,共4页
Chinese Journal of Clinical Anatomy
关键词
异氟醚预处理
OGD损伤
皮层神经元
Isoflurane preconditioning
Oxygen-glucose deprivation
Cortical neurons
