摘要
目的 观察成骨细胞对胚胎干细胞（ESC）向造血前体细胞分化的促进作用.方法 用平皿黏附法培养BALB/c小鼠骨髓基质细胞,诱导分化为成骨细胞,鉴定后用作支持细胞.将小鼠胚胎于细胞（ESC-D3）先诱导生成胚胎体（EB）,再与成骨细胞共培养,3d后进行代表血液血管干细胞的原始细胞集落（BL-CFC）培养并计数;7d后行流式细胞仪检测ESC来源的CD34＋细胞生成比例.结果 在成骨细胞的支持下,与空白对照比较,ESC来源的代表血液血管干细胞的BL-CFC集落增加了2.89倍;由ESC生成的CD34＋细胞由（12.68±2.39）％增加到（32.83±3.84）％.结论 成骨细胞体外可促进胚胎干细胞向造血前体细胞分化.
Objective To explore the role of osteoblasts in promoting the differentiation of embryonic stemcells （ESCs） into hematopoietic precursor cells.Methods The cultured bone marrow BALB/c mouse stromal cells using plating adhesion method were induced to differentiate into the osteoblasts which were identified and used as the supporting cells of subsequent experiments.The murine ESCs-D3 were induced to become the embryonic body （EB）,then co-cultured with the osteoblasts.Three days later,the blast colony-forming cells （BL-CFCs） representing hemangioblasts cultures was done.Seven days later,the ESCs-derived CD34 ＋ cells were detected by using flow cytometry.Results Supported by osteoblasts,as compared with the blank control,the number of ESCs-derived BL-CFCs （hemangioblasts） was increased by 2.89 times and the ratio of ESCs-derived CD34＋ cells was increased from （12.68 ± 2.39）％ to （32.83 ± 3.84） ％.Conclusion Osteoblasts could promote the differentiation of ESCs into hematopoietic precursor cells in vitro.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第12期2649-2651,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81300387）
关键词
成骨细胞
造血
胚胎干细胞
造血前体细胞
Osteoblasts
Hematopoiesis
Embryonic stem cells
Hematopoietic precursor cells
