期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

一株能降解牛奶中过敏原αs1-酪蛋白的蛋白酶产生菌的筛选、鉴定及免疫学研究

Screening of a strain producing protease priority of digest allergen protein(α s1-casein) in milk and its immunology research
在线阅读 下载PDF
导出
摘要 从牛奶场附近土壤中筛选得到1株能降解牛奶过敏原蛋白(αs1-酪蛋白)的菌株Exiguobacterium BBE201110,能产分子量约为55 kDa的蛋白酶,酶活达到346.48 U/mL,对该酶降解牛奶过敏原(αs1-酪蛋白)效果进行免疫学研究(Western blot)表明,在牛奶中添加4%的酶溶液进行降解20 min,酪蛋白特异性清除显著,过敏原蛋白αs1-酪蛋白浓度明显的降低。本实验筛得降解牛奶过敏原蛋白(αs1-酪蛋白)的菌株并成功纯化出蛋白酶,具有一定的食品工业开发价值及商业应用潜力。 In this study, the Exiguobacterium BBE201110 was isolated from dairy farm soil. One of its protease with the molecular mass of approximately 55 kDa and its activity as high as 346.48 U/mL was confirmed by Western blot to digest allergen protein （alpha s1-casein） in milk specifically. In this case, this study provided a novel way to develop nutritious hypoallergenic milk products which might be used in the field of hypoallergenic foods.
作者 谈铭 张娟 朱婷 堵国成 陈坚
机构地区 食品科学与技术国家重点实验室 江南大学生物工程学院 糖化学与生物技术教育部重点实验室 粮食发酵工艺与技术国家工程实验室
出处 《工业微生物》 CAS CSCD 2013年第6期60-67,共8页 Industrial Microbiology
基金 致敏食品脱敏关键技术研究及相关装备研制(2011BAK10B03)
关键词 αs1-酪蛋白 Exiguobacterium BBE201 1 10 免疫验证 alpha sl-casein Exiguobacterium Western blot
分类号 TQ925.2 [轻工技术与工程—发酵工程]
  • 相关文献

参考文献15

  • 1Nowak-Wegrzyn A , Sampson HA. Future therapies for food al- lergies. J Allergy Clin Immunol, 2011,127 (3) :558-575.
  • 2Archimbaud C, Chambon M, Bailly JL, et al. Impact of Rapid Enterovirus Molecular Diagnosis on the Management of Infants, Children, and Adults with Aseptic Meningitis. J Medi Viral, 2009,81 ( 1 ) : 42-48.
  • 3Monaci L,Tregoat V, VanHengel AJ, et al. Milk allergens, their characteristics and their detection in food: a review. Eur Food Res Technol, 2006,223(2) : 149-179.
  • 4Exl BM, Fritsche R. Cow' s milk protein allergy and possible means for its prevention. Nutrition, 2001,17(7-8) : 642-651.
  • 5赵红霞.水解蛋白婴儿配方奶粉的研究[J].乳业科学与技术,2011,34(3):112-113. 被引量：8
  • 6Wal JM. Structure and function of milk allergens. Allergy, 2001, 56(s67) : 35-38.
  • 7Besler M, Steinhart H, Paschke A. Stability of food allergens and allergenieity of processed foods. Journal of Chromatography B: Biomedical Sciences and Applications, 2001, 756 (1-2): 207- 228.
  • 8Kim HJ , Natori Y. Purification and characterization of an alka- line pretease from rat liver. Tokushima J Exp Med, 1982, 29( 1- 2) : 117-23.
  • 9Lindberg RA, Eirich LD ,Price JS, et al. Alkaline protease from Neurospora erassa. Purification and partial characterization. J Bi- ol Chem, 1981,256(2) : 811-814.
  • 10Alaimo M, Farrell Jr HM, Germann M. Conformational analysis of the hydrophobic peptide etsl-casein (136-196). BBA, 1999,1431 (2) : 410-420.

二级参考文献5

  • 1SINDAYIKENGERASeverin.牛乳蛋白改性及水解产物在配方乳中应用研究[D].博士学位论文.
  • 2SALOR J D, BA HNA S L. Anaphylaxis to casein hydrolysate formula [J]. J Pediatr, 1991, 118: 71.
  • 3HEDDLESON R A, PARKO, ALLEN J C.I mmunogemcity of casein phosphopeptides derived from tryptic hydrolysis of casein [J]. J Dairy Sci., 1997, 80: 1971-1976.
  • 4NIGGE MANN B, BINDER C, KLETTLE U, et al. In vivo and in vitro studies on the residual allergenicity of partially hydrolyzed infant formulae[J]. Acta Paediatr, 1999, 88:394 -398.
  • 5BIANCA M E. A review of recent development in the use of moder-ately hydrolyzed whey formulae ininfant nut rition [J]. Nutr Res., 2001, 21: 355+379.

共引文献7

工业微生物
2013年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部