摘要
在Micro Vax II及IBM/PC机上用Framis,Genepro软件,蛋白质、核酸序列数据库Swissprot,PIR,EMBL和Genbank,比较了H-1或MVM的四种蛋白质与现已报道的致癌蛋白质序列的同源性。结果表明,它们之间的配对百分数很低且是随机的,这可能是细小病毒没有致癌潜力的佐证。本实验室还初步证明PV MVM可以杀死经人Ha-ras与v-myc共转染大鼠胚胎成纤维细胞形成的转化细胞,这将为临床治疗抗放射线的人癌提供新途径。
In the in vitro studies, the parvoviruses (PVs) H-1 and MVM showed inhibi-
tory or killing effects on transformed cells and cancer cells. In PVs, there were 2
capsid proteins, VP_1 and VP_2, and 2 non-structural proteins, NS_1 and NS_2, the last
two were reported to have cytotoxic effect on transformed cells. In the field of
chemotherapy and radiotherapy, it is assumed that most of the physical or chemical
agents have both the therapeutic and oncogenic effects depending on the doses. In this
study, using computer Micro Vax II and IBM/PC, Softwares Framis and Genepro,
Protein and Nucleic Acid Sequence Databases Swissprot, PIR, EMBL and Genbank,
the sequence homology of all four proteins of H-1 or MVM were searched and
compared with the oncoproteins reported to date. The results showed that the match%
among them are very low and stochastically perfect. It may affords an eligible
evidence that the PVs have no oncogenic potency. Another known fact was that
some patients suffering from cancers are refractory to radiotherapy, reminiscent
of actions related to oncogenes such as ras and myc; preliminary experiment in our
laboratory provided an evidence that PV MVM could kill rat embryo fibroblast
(REF) transformed by human Ha-ras co-transfected with v-myc. This suggests a
future new approach for curing radio-resistant human cancers in the clinics.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1991年第4期405-412,共8页
Journal of Fudan University：Natural Science
基金
国家教委博士点基金
关键词
病毒
蛋白质序列
同源性
致癌
virus
protein sequence
homology
oncogenic
parvovirus
oncoprotein.
