摘要
目的检测三阴乳腺癌(TNBC)组织中C-myc和Skp2的表达情况及其意义。方法选取2008年1月-2011年12月手术切除的TNBC标本56例,采用免疫组织化学链霉素抗生物素蛋白-过氧化物酶连结法检测它们和30例癌旁正常乳腺组织中C-myc和Skp2的表达情况。结果 TNBC组织中C-myc和Skp2表达定位于细胞核,其表达率分别为57.14%(32/56)和58.93%(33/56),明显高于癌旁正常组织(P<0.05);56例TNBC组织中,C-myc和Skp2蛋白在组织学分级Ⅲ级表达率高于Ⅰ+Ⅱ级(P<0.05);C-myc和Skp2的病理分期Ⅲ期的表达率高于Ⅰ-Ⅱ期(P<0.05);表达率高低与腋窝有无淋巴结转移有关(P<0.05),而与病理组织类型无关(P>0.05);相关性分析显示,C-myc和Skp2二者之间呈正相关(P<0.05)。结论 C-myc和Skp2在TNBC中呈明显高表达,与TNBC的发生、发展和转移密切相关。
Objective To investigate the expression of C-myc and Skp2 in triple-negative breast cancer （TNBC）, and to discuss its clinical pathological significance. Methods From January 2008 to December 2011, by using streptavidin-perosidase immunohistochemistry method, the expression of C-myc and Skp2 in 56 TNBC cases and 30 breast tissue adjacent to cancer eases were examined. Results The expression of C-myc and Skp2 in human breast cancer was located within the nucleus. The positive expression rates of C-mye and Skp2 were 57.14% （32/56） and 58.93% （33/56） respectively in TNBC which were higher than that in the adjacent normal tissue （P 〈 0.05）. In the 56 cases of TNBC, the positive expression of C-mye and Skp2 in histological grade G3 was significantly higher than that in G1 and G2 （P 〈 0.05）. In addition, the positive expression of C-mye and Skp2 in stage III - IV tumors was also higher than that in stage I - II tumors （P 〈 0.05）. Furthermore, the expression of C-myc and Skp2 were associated with axillary lymph node metastasis （P 〈 0.05）, and were not related with histopathologieal type （P 〉 0.05）. Correlation analysis showed a positive correlation between C-myc and Skp2 （P 〈 0.05）. Conclusion The high expression of C-myc and Skp2 are found in TNBC, which are closely related with the occurrence, development and metastasis of TNBC.
出处
《华西医学》
CAS
2013年第11期1675-1677,共3页
West China Medical Journal
