摘要
目的为了解水族和仡佬族HLA-G基因14bp插入/缺失多态性与其遗传背景之间相互关系。方法采用PCR扩增及测序的方法,对来自贵州地区水族和仡佬族340例健康个体的HLA-G 14bp插入/缺失进行检测和分析。并采用毛细管电泳的方法,对这两个群体各40人的10个微卫星位点进行了分型,用于分析这两个群体间的遗传关系。结果用10个微卫星对遗传背景的分析表明,这两个群体的遗传关系较近。而14 bp插入等位基因频率存在差异(14 bp插入等位基因频率:水族,0.455;仡佬族,0.336;经χ2检验,P=0.0016)。并且根据10个微卫星位点所绘制的群体系统发育树与根据14bp所绘制的基因树也存在较大差异。结论群体的遗传背景与14bp的分布频率并不一致。考虑到HLA在进化中可能受到的平衡选择作用,群体中14bp的频率分布可能不仅仅是由于遗传背景所决定的,其他因素如自然选择可能打破了遗传背景对HLA-G14bp的插入/缺失多态性分布的影响。
Objective： To investigate the relationship between HLA - G gene 14bp insertion/deletion ploymorphism and genetic background in Shui and Gelao population. Methods： PCR amplification and sequencing are used to explore the distribution of 14bp in- sertion/deletion in HLA - G gene in 340 people of these two ethnic groups. In the mean while, in order to assess the genetic relation- ship between the Shui and Gelao people, we genotyped 10 mierosatelites by capillary electrophoresis in 80 people in these two ethnic groups. Results： The results of 10 microsatellites demonstrate that the two ethnic groups have a similar genetic background. However, the frequencies of 14bp insertion/deletion is statistically different in the two ethnic groups （the frequences of 14bp insertion are as fol- lows： Shui people 0. 455 ; Gelao, 0. 336; X2 = 10. 01, P =0. 0016）. Conclusion： These results suggested that the distribution of 14bp insertion/deletion in different populations does not always have accordance with their genetic backgrounds. Considering the balancing selection during HLA evolution, especially for the southern populations under a strong selection pressure in China, we propose that the balancing selection maybe break the connections between distributions of 14bp and genetic backgrounds in these populations.
出处
《中国优生与遗传杂志》
2013年第11期11-14,6,共5页
Chinese Journal of Birth Health & Heredity
基金
国家高技术研究发展计划(863)项目(编号:2012AA021802)
贵州省科学技术基金编号:黔科合J字(2012)2211
协和青年基金编号:3332013085
