摘要
目的观察补骨脂水煎剂对去卵巢肥胖大鼠体重和肿瘤坏死因子-α(TNF-α)的影响。方法将SD大鼠随机分为:假手术组、手术组和补骨脂组。手术组和补骨脂组行双侧卵巢切除术,假手术组只切除卵巢周围同卵巢大小相似的脂肪组织观察补骨脂水煎剂对去卵巢肥胖大鼠的体重、摄食量和饮水量的影响。术后55 d,股动脉取血并测定血糖、胰岛素、雌激素、白细胞介素-6(IL-6)和TNF-α水平,并测量其体长,计算Lee's指数。结果双侧卵巢切除术后,手术组体重、摄食量、TNF-α水平和Lee's指数均高于假手术组(P均<0.01)。饮用补骨脂水煎剂后,补骨脂组的体重、摄食量、TNF-α水平和Lee's指数均低于手术组(P均<0.05)。结论补骨脂水煎剂通过抑制摄食达到降低去卵巢肥胖大鼠体重的作用。
Objective To observe the effects of water extracts from Fructus Psoraleae on the weight and level of tumor necrosis fac tor-α （TNF-α） in ovariectomized rats. Methods Adult female SD rats weighted （220 ± 3）g were randomly divided into three groups with 8 rats in each group： sham-operated, ovariectomized and Fructus Psoraleae groups. The rats were underwent a bilateral ovariectomy except those in the sham-operated group which were subjected to the removal of bilateral fat as much as ovariectomized group with the ovaries re mained. The rats in sham-operated and ovariectomized groups freely drank water while the rats in Fructus Psoraleae group freely took 1% wa ter extracts from Fructus Psoraleae and the concentration of Fructus Psoraleae gradually increased to 12% which was continued for the end of the experiment （55 days）. The food-intake and water-intake of the rats in each group were weighed daily and the body weight was measured every five days. At the end of the experiment, femoral artery blood samples of the rats were collected to determine the levels of plasma glu cose, insulin and estrogen. At the same time, the levels of interleukin-6 and TNF-α were measured according to the kits. Finally Lee＇s index was calculated by measuring their body length. Results The weight, food-intakes, Lee＇s index and the level of TNF-α in ovariectomized group were higher than those in sham operated group （P 〈0. 001 ,P 〈0. 001 ,P 〈0. 01 ,P 〈0. 001 respectively）. After the rats in Fructus Psoraleae group drank the water extracts from Fructus Psoraleae, their weight, food-intake, level of TNF-α and Lee＇s index were lower than those of the rats in ovariectomized group （P 〈 0. 001, P 〈 0.05, P 〈 0.01, P 〈 O. 05 respectively）. Conclusions The water extracts from Fructus Psoraleae could reduce the body weight of ovariectomized rats by inhibiting food-intake.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2013年第23期5886-5888,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金资助项目(No.30572428)
关键词
补骨脂水煎剂
去卵巢大鼠
肥胖
肿瘤坏死因子-Α
Water extracts from Fructus Psoraleae
Ovariectomized rats
Obesity
Tumor necrosis factor-α
