摘要
运用超低温 (- 1 96℃ )保存手段 ,通过对锥栗种子离体胚超低温保存后脱氢酶活性的方差分析和 Q检验法多重比较分析 ,对其长期保存的可行性进行研究 ,结果表明 :含水量是影响锥栗种子离体胚超低温保存的重要因素 ,超低温保存应进行适度脱水 .无防冻剂预处理 ,2 0 %含水量 ,缓冻缓解方式条件下 。
By applying cryopreservation(-196 ℃)treatment and using the methods of variance analysis and multiple comparison of Q test of TTCH content of excised embryos after cryopreservation, dehydrogenase activity of excised embryos was analysed and long term storage feasibility was studied. The results showed moisture content(MC) was the main factors affecting the cryopreservation of C. henryi excised embryos, and the measures desiccating a little down to a medium MC should be taken in cryopreservation. Under the conditions of no cryoprotectants pretreatment, 20% MC, mild freezing, and quick thawing, the dehydrogenase activity of excised embryos was the highest.
出处
《福建林学院学报》
CSCD
北大核心
2001年第1期32-35,共4页
Journal of Fujian College of Forestry
基金
国家自然科学基金资助项目! (396 70 6 0 8)
关键词
锥栗
种子贮藏
离体胚
超低温保存
脱氢酶活性
Castanea Henryi
Seeds storage
Cryopreservation
Excised Embryos
Dehydrogenase activit
