摘要
目的参考联合国粮农组织/世界卫生组织(FAO/WHO)食品添加剂联合专家委员会推荐的方法,在对包括纸片规格、培养基、标准菌株浓度、阳性对照物浓度等影响酶制剂抗菌活性检测主要因素优化的基础上,建立微生物源酶制剂抗菌活性检测方法。方法以浓度为5.0和10.0μg/片的环丙沙星纸片为阳性对照,在相同的培养温度、培养时间等试验条件下,比较9种不同规格纸片、3种品牌培养基、3个菌液稀释度对6种标准菌株抗菌活性的影响。结果 HZT 2号滤纸、2张叠加的whatman903纸片与商业化抗菌活性测定用纸片Whatman 2017-013效果相当;国产1号和2号胰蛋白胨大豆琼脂(TSA)与进口TSA效果相当;确定5种受试菌培养24 h后菌液的10倍稀释液为工作浓度(化脓链球菌的工作浓度为菌液的20倍稀释液);环丙沙星纸片的工作浓度为5.0μg/片。结论HZT 2号滤纸、2张叠加的Whatman903滤纸或Whatman 2017-013滤纸、国产或进口TSA培养基、受试微生物培养24 h的10倍(化脓链球菌20倍)菌液稀释液、环丙沙星浓度5.0μg/片为微生物源酶制剂抗菌活性测定中优化出的最佳试验条件。
Objective To develop an antimierobial activity detection method of enzyme preparations derived from microorganism, the key factors including paper specification, medium, the concentration of microorganisms as well as positive control antibiotics were screened and examined, based on the method recommended by JECFA. Methods Taking 5.0 and 10.0μg/piece of ciprofloxacin as positive control, nine kinds of paper, three kinds of medium and three bacteria concentrations under the same conditions （temperature, incubation time, etc ） were checked for their influence on antimicrobial activity. Results The effect of HZT No. 2 and Whatman 903 （2 piece superposed） were comparable with the commercialized antimicrobial determining paper. The effect of tryptone soy agar （TSA） domestically produced （defined as No. 1 and No. 2） were comparable with imported TSA. The appropriate dilution of tryptone soy broth to 24 h cultures was 10 ： 1 for 5 reference stains, and 20 ： 1 for Streptococcus pyogenes. The recommended concentration for ciprofloxacin antimicrobial activity test was 5.0 μg/piece. Conclusion Paper HZT No. 2 or Whatman903 （2 piece supcrposed） or whatman 2017-013, domestic or imported TSA medium, 10 times culture dilution （20 times for Streptococcus pyogenes）, and 5.0 μg/piece ciprofloxacin were recommended in the determination of antimicrobial activity of enzyme preparations derived from microorganism.
出处
《中国食品卫生杂志》
北大核心
2013年第6期515-519,共5页
Chinese Journal of Food Hygiene
基金
卫生部标准修订项目(spaq-2011-60)
关键词
酶制剂
抗菌活性
抗菌素
微生物
滤纸片
影响因素
Enzyme preparations
antimierobial activity
antibiotics
microbe
filter paper
effect factors
