摘要
为了测定球孢白僵菌对不同生境淡色库蚊幼虫的致病力，为白僵菌应用于蚊虫的生物防治提供参考依据，从济宁市唐口镇医院污水（A组）、市郊区养猪场废水沟（B组）、微山湖稻区排水沟（C组）、山东省寄生虫病防治研究所院内水缸（D组）中采集的蚊虫直接用于致病力研究，室内饲养蚊虫作为对照组（E组）。利用白僵菌的孢子悬浮液处理不同栖境的淡色库蚊幼虫，记录幼虫死亡数，计算各处理组幼虫的致死中时LT50.结果显示，经白僵菌处理后，淡色库蚊幼虫均有不同程度的死亡率，试验10d后，淡色库蚊幼虫的累计死亡率均达95％以上。A、B、C、D、E组幼虫的致死中时LT50分别为：6．2、6．4、5．7、5．8、3．8d，野外采集蚊虫比室内饲养蚊虫的死亡高峰期推迟2～3d。结果表明白僵菌有望应用于蚊虫的生物防治中。
The present study attempts to analyze the virulence of Beauveria bassiana on Culex pipiens paUens from different habitats, such as sewage at Tangkou Town hospital （A）, polluted piggery water at the outskirts of Jining City （B）, drainage ditch of paddy field in Weishan Lake （ C）, vats in Shandong Institute of Parasitic Diseases （D） and laboratory （E） , and to provide a theoretical basis for biological control of mosquitoes. Spore suspension of B. bassiana was used to process the larvae of Cx. pipiens paUens collected from different habitats. The dead number of larvae was recorded and the median lethal time （ LT50 ） was calculated. The larval mortality was more than 95% 10 d after treatment by B. bassiana. The LTs0 values of A, B, C, D and E were 6. 2 d, 6.4 d, 5.7 d, 5.8 d and 3.8 d, respectively. The LT50 of larvae reared in laboratory was lower. Under laboratory conditions, B. bassiana showed efficient control effect on Cx. pipiens pallens collected from different habitats. The peak value of dead larvae reared in laboratory was 2-3 days in advance than those collected outdoors. It is expected to use B. bassiana in the biological control of mosquitoes.
出处
《寄生虫与医学昆虫学报》
CAS
2013年第3期158-161,共4页
Acta Parasitologica et Medica Entomologica Sinica
基金
山东省科技发展计划项目（2010GSF10207）
山东省自然科学基金项目（ZR2009CQ029,ZR2010HQ062）
关键词
淡色库蚊
球孢白僵菌
致病力
Culex pipiens paUens
Beauveria bassiana
Pathogenicity
