摘要
目的:研究高频重复经颅磁刺激(rTMS)对脑缺血大鼠缺血侧海马BDNF、VEGF和Nestin表达的影响。方法:SD雄性成年大鼠16只,随机分为模型组、rTMS组、rTMS+生理盐水(NS)组和rTMS+H89组各4只,rTMS+NS组和rTMS+H89组分别侧脑室注射NS和H89,4组均建立大脑中动脉栓塞(MCAO)模型,除模型组外均给予7 d 20 Hz rTMS治疗,应用Western Blot方法检测缺血侧海马pCREB、BDNF、VEGF和Nestin表达。结果:rTMS组pCREB、BDNF、VEGF和Nestin表达较模型组均增加(P<0.05),rTMS+H89组pCREB、BDNF、VEGF和Nestin表达较rTMS+NS组均减少(P<0.05)。结论:高频rTMS能使脑缺血后海马区BDNF、VEGF表达增加,并进一步促进Nestin表达,PKA-CREB通路的调节可能是其机制之一。
Objective： To investigate the effects of high-frequency repetitive transcranial magnetic stimulation （rTMS） on the expression of BDNF, VEGF and Nestin in the ischemic hippocarnpus of rats with cerebral is- chemia. Methods： A total of 16 SD rats were randomly divided into model group, rTMS group, rTMS ＋H89 group and rTMS＋normal saline（NS） group （n=4 in each group）. The rats in rTMS＋H89 group and rTMS＋NS group were intracerebroventricular injected with H89 or NS respectively. Reperfusion models with middle cere- bral artery occlusion were established in the 4 groups, rTMS of 20 Hz was given to 3 groups except model group for 7 days. The expression changes of BDNF, VEGF and Nestin were investigated between model group and rTMS group, rTMS ＋H89 group and rTMS ＋NS group by Western Blot respectively. Results： Compared with model control group, the expressions of pCREB, BDNF, VEGF and Nestin were increased in the rTMS group （P〈0.05）. Compared with rTMS＋NS group, the expressions ofpCREB, BDNF, VEGF and Nestin were signifi- cantly decreased in the rTMS ＋ H89 group （P〈 0.05）. Conclusion： Repetitive transcranial magnetic stimulation of 20Hz could promote BDNF and VEGF expressions in the ischemic hippocampus after cerebral ischemia, which may contribute to the neurogenesis.
出处
《神经损伤与功能重建》
2013年第6期431-434,共4页
Neural Injury and Functional Reconstruction
基金
教育部高等学校博士学科点专项科研基金(No.20090142110013)
国家自然科学基金(No.81071601)
关键词
脑缺血
重复经颅磁刺激
CAMP反应元件结合蛋白
脑源性神经营养因子
血管内皮生长因子
巢蛋白
cerebral ischemia
repetitive transcranial magnetic stimulation
cAMP response element bindingprotein
brain-derived neurotrophic factor
vascular endothelial growth factor
nestin
