摘要
目的 :确定长期芦沙坦治疗能否逆转自发性高血压大鼠(SHR)心肌纤维化。方法 :本实验选用 33周龄Wistar Kyoto(WKY)大鼠 8只 ,SHR未用药组 6只 ,SHR用药组 6只 (已口服 17周芦沙坦 30mg/kg/d)。大鼠断头处死 ,取出心脏标本进行检测。血压、心脏重量、左心室重量、左心室重量指数测定用常规方法。纤维胶原用左心室心肌组织切片行VanGieson染色 ,图象用真彩色图象分析系统计算左室胶原容积分数 ,进行定量评估。免疫组化染色左心室心肌组织切片Ⅲ型胶原 ,用记分法进行半定量分析。电镜观察左心室心肌间质形态学变化。用 3× 10 0 0放大比例计算每个视野成纤维细胞数 ,每个标本随机取 10个视野 ,比较各组间成纤维细胞数的差异。结果 :与WKY组大鼠相比较 ,SHR存在高血压、心肌肥大、左心室胶原容积分数增加。免疫组化分析左心室Ⅲ型胶原增加 ,电镜观察成纤维细胞数增加 ,有统计学显著性差异。与SHR组相比 ,SHR用药组高血压、左心室重量、左心室胶原容积分数降低。免疫组化分析左心室Ⅲ型胶原减少。但成纤维细胞数无明显变化。结论 :芦沙坦在逆转自发性高血压大鼠心肌肥大 ,同时能减少基质胶原沉积。但电镜形态学观察 ,不能抑制成纤维细胞增殖。
Objective:To investigete chronic treatment with angiotensionⅡ type 1 receptor antagionist losartan reverses left ventricular fibrosis in SHR. Methods:This study was performed in 33 week old Wistar kyoto(WKY)rats(\%n\%=8), SHR(\%n\%=6), and SHR treated with losartan(30 mg/kg per day)for 17 weeks(\%n\%=6).Blood pressure,Cardiac mass,left ventricular mass and left ventricular mass index were measured.Cardial total collagen was stained with Van Gieson methold.Collage volume fraction was determined in the left ventricle by quantitative morphometry. Collagen Ⅲ stained by immunohistochemistry was evaluated with scoring.Cardiac extracellular matrix was observated with eletromicroscope and the numbers of fibroblasts in left ventricle were calculated. Results:Compared with WKY rats, SHR exhibited increased BP, left ventricular mass,left ventricular collagen volume fraction,left ventricular collagen Ⅲ, and the numbers of cardiac fibroblast(\%P\%<0 05 respectively). After the treatment treated SHR showed no left ventricular hypertrophy and diminished the high BP(\%P\%<0 05). Left ventricular collagen volume fraction and collagen Ⅲ dereased in treated SHR then in untreated SHR,but no changes in numbers of fibroblast were observed between treated SHR and untreated SHR. Conclusion:Chronic treatment with losartan can normalized blood pressure and reversed left ventricular hypertrophy in SHR,significantly inhibited collagen compositions in left ventricle. But it cannot inhibit proliferation of cardiac fibreblasts in SHR.
出处
《高血压杂志》
CSCD
2000年第4期345-348,共4页
Chinese Journal of Hypertension
关键词
芦沙坦
治疗
高血压
心肌间质
losartan
spontaneously hypertensive rats
myocardiac hypertrophy
extracellular matrix fibrosis
