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微囊藻毒素-LR的分离纯化

Isolation and Purification on Microcystin-LR
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摘要 微囊藻毒素-LR(MC-LR)是淡水蓝藻产生的毒性极强的毒素,通过接触、饮用等途径对人体健康造成极大威胁。通过对产微囊藻毒素-LR的铜绿微囊藻进行培养,采用超声波破碎、溶剂萃取法进行萃取,并经高效液相色谱法进行纯化,最终获得微囊藻毒素-LR纯品,为微囊藻毒素-LR的进一步研究奠定基础。 Microcystin-LR (MC-LR) is a highly toxic substance produced by freshwater cyanobacteria. There is a great threat to human health through contacting or drinking it. Microcystis aeruginosa produced MC-LR were cultured to some extent and the algae cells were crushed by ultrasonic. Then solvent extraction methods and high performance liquid chromatography (HPLC) methods were carried out. MC-LR was got finally after freeze-drying. It lays the foundation for further research on MC-LR.
出处 《长春理工大学学报(自然科学版)》 2013年第5期161-163,共3页 Journal of Changchun University of Science and Technology(Natural Science Edition)
基金 吉林省科技厅社会发展计划项目(20100407)
关键词 铜绿微囊藻 微囊藻毒素-LR 溶剂萃取 高效液相色谱 microcystis aemginosa microcystin-LR solvent extraction high performance liquid chromatography (HPLC)
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参考文献5

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  • 3CHEN J,XIE P. Tissue distributions and seasonaldynamics of the hepatotoxic microcystins—LRand —RR in two freshwater shrimps, palaemonmodestus and macrobrach iumnipponensis, from alarge shallow, eutrophic lake of the subtropical China[Jj.Toxicon,2005,45(5):615-625.
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