摘要
目的探究海地瓜硫酸软骨素(Acaudina Molpadioides chondroitin sulfate,AM-CHS)抑制3T3-L1前脂肪细胞分化的分子机制。方法采用传统的鸡尾酒诱导剂诱导分化3T3-L1前脂肪细胞,分别采用油红O染色和甘油三酯(Triglycerides,TG)含量测定法评价AM-CHS对3T3-L1前脂肪细胞分化的影响。采用RT-PCR法检测脂肪细胞中Wnt信号通路关键基因和脂肪合成关键基因mRNA的表达水平。结果AMCHS能够显著抑制3T3-L1细胞脂滴的形成,拮抗罗格列酮(Rosiglitazone,RSG)的促分化作用,并上调Wnt信号通路中的关键受体Frz和LRP5的mRNA表达,下调分化转录因子PPARγ、C/EBPα和SREBP-1cmRNA的表达,抑制脂质合成基因FAS和GPAT mRNA的表达,不影响脂质分解基因HSL和ATGL mRNA的表达。结论AM-CHS能够显著抑制3T3-L1前脂肪细胞的分化,抑制成熟脂肪细胞的脂质合成,并拮抗罗格列酮(Rosiglitazone,RSG)的促脂质合成作用,其作用机制与激活Wnt信号通路有关。
Objective To investigate the mechanism of AcaudiTla Molpadioides chondroitin sulfate on the suppression effect of 3T3-L1 preadipocytes differentiation. Methods After using differentiation cocktail to differentiate 3T3-L1 preadipocytes, the effect of AM-CHS on the differentiation of 3T3-L1 preadipo-cytes was carried out by oil red O staining and TG content determination. Expression levels of key genes in Wnt signal passway and lipogenesis were measured by reverse transcription PCR（RT-PCR）. Results AM-CHS observably inhibited the form of lipid droplet in 3T3-L1 mature adipocytes and re versed the promoting differentiation effect of Rosiglitazone（RSG）. Besides, AMCHS could up regulate the expressions of Frz and LRP5 rnRNA which were key receptors in Wnt signal passway. Meanwhile AM-CHS observably down-regulated the expression of PPARTγ、 C/EBPα. SREBP-lc. FAS and GPAT mRNA, but showed no effect on the expression of HSL and ATGL mRNA. Conclusion AM-CHS could markedly suppress the differentiation of 3T3-L1 preadipocytes, inhibite lipid accumulation of 3T3-L1 mature adipocytes and reverse the promoting lipogenesis effect of Rosiglitazone（RSG） by activating the Wnt signaling pathway.
出处
《中国海洋药物》
CAS
CSCD
北大核心
2013年第6期49-57,共9页
Chinese Journal of Marine Drugs
基金
海洋公益性行业科研专项(201105029)
"十二五"国家科技支撑计划(2012BAD33B07)
长江学者和创新团队发展计划资助
