摘要
目的观察慢性心理应激对2型糖尿病(T2DM)小鼠糖脂代谢及胰岛素抵抗(IR)的影响及加减逍遥散的作用效果。方法 102只自发性糖尿病(KK)小鼠随机分为5组:正常对照(WC)组,模型(SC)组,加减逍遥散对照(XYS)组,应激+加减逍遥散低剂量(LXYS)组及应激+加减逍遥散高剂量(HXYS)组。KK小鼠用高糖高脂饲料喂养8周,采用电击、夹尾、禁食、禁水、合笼饲养等方法复制慢性心理应激模型。检测各组小鼠空腹血糖(FPG)、血清胰岛素(FINS)以及血脂的胆固醇(CHO)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)4项等指标,计算胰岛素敏感指数(ISI)。结果与WC组比较,SC组的ISI明显降低(P<0.01),体质量明显下降(P<0.01),XYS组的FPG明显降低(P<0.01)、ISI明显增强(P<0.05);与SC组比较,LXYS组及HXYS组的ISI明显增强(P<0.01),HXYS组体质量明显升高(P<0.05),TG,CHO,LDL-C,明显降低(P<0.01,P<0.05),HDL-C,明显升高(P<0.05)。结论慢性心理应激能降低T2DM小鼠的胰岛素敏感性,从而加剧其IR以及糖脂代谢的紊乱;加减逍遥散不仅能提高T2DM小鼠的胰岛素敏感性,减轻IR,同时也能对抗慢性心理应激对T2DM小鼠IR的不良影响。
Objective To observe the influences of chronic psychological stress on glycolipid metabolism disorder and insulin resistant （IR） of type 2 diabetes mellitus （T2DM） mice and the intervention effect of Modified Xiaoyao San （XYS）. Methods One hundred and two KK mice were randomly divided into 5 groups, normal control group （WC group）, model group （SC group）, T2DM mice treated with XYS group （XYS group）, stress mice treated with low-dose XYS group （LXYS group） and stress mice treated with high-dose XYS group （HXYS group）. The KK mice model of T2DM was induced by feeding high-glucose and high-fat forage for 8 weeks, and the model of chronic-psychological- stress was induced by electric shock, clamping the tail, fasting treatment, and raising the male and female mice in the same cage. The observation indexes included fasting plasma glucose （FPG）, fasting insulin （FINS）, total cholesterol （ TC ）, triglyeeride （ TG ）, low-density lipoprotein cholesterol （ LDL-C ）, and high-density lipoprotein cholesterol（ HDL-C ）. At last the insulin sensitivity index（ISI） was calculated. Results Compared with WC group, SC group had low ISI, body weight and FPG but had high ISI（P 〈 0.01 ）. Compared with SC group, S＋LXYS group and S＋HXYS group had low HbAlc but had high ISI （P 〈 0.01 ）, HXYS group had high body weight and HDL-C （P 〈 0.05）, and had low TG, TC and LDL-C（P 〈 0.05 or P 〈 0.01）. Conclusion Chronic psychological stress can reduce insulin sensitivity and make IR and glycolipid metabolisim disorder worse in T2DM KK mice. Modified Xiaoyao San can increase ISI, reduce IR and counteract the harmful influences resulting from chronic nsvchological stress in T2DM mice.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2013年第6期555-557,592,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省中医药强省课题(20121230)
广东省教育厅重点实验室课题(AAF433111A50)
广州中医药大学中医药科研创新基金项目(K0100074)
关键词
加减逍遥散
慢性心理应激
2型糖尿病
胰岛素抵抗
Modified Xiaoyao San
Chronic psychological stress
Type 2 diabetes mellitus
Insulin resistance
