摘要
本实验室从安徽某羊场发生腹泻的山羊病料中检测分离到边界病病毒（borderdiseasevirus，BDV），证明BDV在我国羊群中存在。为了进一步了解BDV在江苏羊群的流行情况，本研究收集江苏部分地区发生腹泻和健康羊群的血清和组织样品，采用RT—PCR方法进行检测，并对阳性样品进行病毒的分离鉴定，测定分离毒株5’-UTR基因片段，与其他已报道的毒株进行同源性比较并绘制进化树。结果表明有27．4％（29／106）样品呈阳性，不N羊场BDV阳性率为0～67％，共分离到4株不同的BDV毒株，它们之间的同源性为73．9％～95．6％，而与其他BDV毒株的同源性为66．2％～91．6％。进化分析表明AH12-02与其他各毒株均较远，形成单独的分支，另3个毒株与BDV3型毒株关系最近。采用ELISA试剂盒对血清样品BDV抗体进行检测发现不同羊场抗体阳性率存在较大差异（0～100%），还有抗体阴性持续感染个体的存在。以上结果丰富了我国BDV分子流行病学数据，为进一步探索BDV在我国羊群中的流行情况奠定了基础。
Border disease virus （BDV） was first detected from a goat herd suffering diarrhea in An- hui province,which confirmed the existing of BDV in chinese goat herds. In order to understand the prevalence status of BDV in goat/sheep herds in Jiangsu province,sera and tissue samples were taken from diseased and healthy herds, and detected for BDV by RT-PCR. Positive samples were further used for virus isolation. 5＇-UTR gene fragments of isolated strains were sequenced and a- ligned with other reported sequences for phylogenetic analysis. The results showed that 27 out of 90 samples were positive and the positive rate from different farm was 0-67%. Four different strains were isolated finally. They shared nucleotide acid identity of 73.9%-95.6% each other and of 66.2%-91.6% with other reported sequences. AH12-02 formed a distinct branch,while the oth- er 3 possessed high relationship with BDV3 genotype strains. Sera samples were tested for BDV specific antibody by commercial ELISA kit and showed varied positive rate （0-100%） at the farms. Some individuals with persistent infection were also found negative. These results provid the epidemiological data of BDV prevalence in China and will be helpful for further exploration ofBDV infection status in Chinese goat herds in the future.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第12期1808-1812,共5页
Chinese Journal of Veterinary Science
基金
江苏省农业科技自主创新资金资助项目(SCX(12)3143)
