摘要
目的研究羧基磁珠吸附乙型肝炎病毒表面抗原(HBsAg)的性能,为磁珠在蛋白质分离纯化中的应用打下基础。方法检测羧基磁珠吸附HBsAg前后的蛋白量,判断磁珠用量、HBsAg浓度、吸附温度、吸附时间对磁珠吸附HBsAg的影响。使用10mmol/L NaOH洗脱HBsAg,测定洗脱后HBsAg的洗脱率和生物活性保留率。结果羧基磁珠吸附HBsAg的性能与磁珠用量、HBsAg浓度、吸附温度和吸附时间有密切关系。磁珠吸附浓度为524.24μg/mL的血源性HBsAg纯品后,10mmol/L NaOH溶液洗脱HBsAg的洗脱率是71.02%,活性保留率为88.15%。结论带有羧基的磁珠可以吸附HBsAg,有望用于HBsAg的分离回收,为磁珠在蛋白质分离纯化中的应用打下基础。
Objective To lay the foundation for the application of magnetic beads in protein purification by re- searching carboxyl magnetic beads adsorption performance of HBsAg. Methods The amount of magnetic beads, HB- sAg concentration,adsorption temperature and the influence of adsorption time by detecting the protein amount be- fore and after adsorption were detected. Results Adsorption HBsAg capacity of magnetic beads was affected by a- mount of magnetic beads, HBsAg concentration, adsorption temperature and adsorption time. After magnetic beads adsorption of HBsAg whose concentration was 524.24 μg/mL, the adsorbed HBsAg could be eluted by 10 mmol/L NaOH,the elution rate was 71.02%, and the retained biological activity was 88.15 %. Conclusion Magnetic beads with carboxyl can adsorption HBsAg,which is expected to be used for the separation and recovery of HBsAg. It has lay the foundation for the application of magnetic beads in protein purification
出处
《检验医学与临床》
CAS
2013年第22期2978-2979,2981,共3页
Laboratory Medicine and Clinic
基金
广东省广州市属高校科研项目(10A204)
