摘要
目的 :研究三氧化二砷 (As2 O3)对慢性粒细胞K5 6 2 (CML) ,急性粒细胞白血病细胞株HL - 6 0 (AMLM2 )的作用并与细胞株NB4(APLM3)作比较。方法 :采用形态学和流式细胞术观察不同浓度As2 O3 对HL - 6 0 ,K5 6 2及NB4细胞的作用。结果 :As2 O3不仅能诱导NB4的凋亡 ,提高其浓度至 2 .7μM和 8.1μM ,也能诱导K5 6 2 ,HL - 6 0的凋亡。As2 O3 不能诱导三种细胞分化。结论 :As2 O3 对细胞诱导的凋亡是非选择性的且不能诱导分化。
Objective:[WT5BZ]To study the mechanism and effect range of arsenic trioxide (As 2O 3) on myelocytic cell lines.[WT5HZ]Methods:[WT5BZ]Light microscope and flow cytometer were used to observe morphologic changes of NB4,K562 and HL-60.[WT5HZ]Results:[WT5BZ]As 2O 3 is able to induce 57.2%,60.0% apoptosis in K562(CML),HL-60(AML M 2) cells respectively at the concentrations of 2.7 μM and 8.1 μM and induces 50.9% apoptosis in NB4(M 3) cell at the concentration of 0.6 μM.[WT5HZ]Conclusion:[WT5BZ]The apoptosis induced by As 2O 3 was non-selective.There was no effect of As 2O 3 on differentiation antigens CD11b,CD14 and CD15. [WT5HZ]
出处
《西北国防医学杂志》
CAS
2000年第4期248-250,共3页
Medical Journal of National Defending Forces in Northwest China
