摘要
为探讨雌激素和异黄酮类药物对内皮细胞粘附分子 CD5 4表达的影响 ,作者采用 10 ng/ ml肿瘤坏死因子 (TNFα)处理人脐静脉内皮细胞 6小时 ,并加用不同浓度的异黄酮 (WZ1 ,WZ2 )和雌激素 (WZ3,WZ4)进行干预 48小时 ,而后用流式细胞仪定量测定细胞表面粘附分子 CD5 4的表达 ,并进行统计学分析。结果 :110 ng/ ml的TNFα可使内皮细胞表面粘附分子 CD5 4的表达升高 4倍 ,与对照组相比具有统计学差异 (P<0 .0 5 ) ;2 10 - 1 0 m ol/L、10 - 8m ol/ L、10 - 6 m ol/ L 异黄酮和异卟黄酮对由 TNFα诱导的内皮细胞的 CD5 4表达有轻度促进作用 ;3哌嗪雌酚酮和雌二醇预处理内皮细胞 48小时后再加 TNFα激活 ,内皮细胞表面 CD5 4平均荧光强度与单纯 TNFα组相比下降明显 (P<0 .0 5 ) ,各浓度组之间不具统计学差异 (P>0 .0 5 )。以上结果提示 :异黄酮和异卟黄酮对内皮细胞粘附分子 CD5 4的表达无抑制作用 ;哌嗪雌酚酮和雌二醇可在一定程度上抑制 CD5 4的表达 ,从而为抗粘附疗法提供了一条新思路。
This study was aimed to elucidate the effects of estradiol and isoflavoid on the expression of CD54 on endothelial cells. 10ng/ml tumor necrosis factor alpha (TNFα) was used to incubate endothelial cells (ECs) for 6h; different concentrations of isoflavoid (WZ 1, WZ 2) and estradiol (WZ 3,WZ 4) were added in respectively and incubated for 48h. Flow cytometry was used to assay the expression of adhesion molecules CD54 on ECs quantitatively. The results showed: ① 10ng/ml TNFα could increase the expression of CD54 on ECs by 4 folds; ② isoflavoid could increase the expression of CD54 on ECs slightly. ③ after the ECs had been incubated with different concentrations of estrogen for 48 h, the expression of CD54 decreased by 15% as compared with that of the TNFα treated group. These findings suggested that isoflavoid had no protective effect on the expression of CD54, but estradiol might inhibit the expression of CD54.
出处
《华西医科大学学报》
CAS
CSCD
2000年第4期445-448,455,共5页
Journal of West China University of Medical Sciences
基金
国家自然科学基金!资助项目 (批准号 396 70 2 0 2 )
卫生部科学研究基金!资助项目 (项目编号 96 -2 -192 )
纽约中华医学基金会部
