摘要
前单核细胞 THP- 1分化是一个复杂的过程 ,这一过程包含了复杂的细胞内信号传导及许多因子的活化及大量蛋白的表达。基质金属蛋白酶 - 9(Motrix Metalloproteinase- 9,MMP- 9)主要降解基底膜中的 和 型胶原 ,在血管增生及肿瘤浸润转移过程中起重要的作用。为探讨核转录因子 κB(nuclear factor kappaB,NF- κB)在 THP- 1粘附及 MMP- 9表达中的作用 ,我们应用 NF- κB抑制剂 PDTC(Pyrollidine Dithiocarba-mate二硫碳吡咯烷 ,应用共聚焦显微镜、流式细胞术观测了其对 THP- 1粘附及 MMP- 9表达的影响。结果表现 :PDTC可明显的抑制 THP- 1的粘附及 MMP- 9的表达。表明 NF- κB在细胞粘附及 MMP- 9的表达中起重要的作用。
The differentiation of pro monocyte THP 1 is a complicated process which includes the activation of many transcription factors such as NF κB and the cxpression of many proteins such as adhesion molecules. In the article, we use the inhibitor of NF κB, pyrollidine dithiocarbamate (PDTC) to explore the effect of PDTC on the THP 1 adhesion and MMP 9 expression measured by flow cytometry. The results suggest that the inhibitor of NF κB inhibits the adhesion of THP 1 and partly inhibits MMP 9 expression.
出处
《解剖科学进展》
CAS
2000年第4期363-365,共3页
Progress of Anatomical Sciences
