摘要
利用3种不同的毒素活性检测方法 (离体叶片针刺法、根冠细胞染色法和游离原生质体染色法)检测玉米小斑病菌C小种(HMC)的毒素生物活性,比较其检测效果,用以探讨HMC毒素致病机理。结果表明:离体叶片针刺法虽能简便地测出毒素的活性,但不能做定量分析,仅从宏观上检测出病斑的形成。根冠细胞染色法,不仅定性还可定量分析毒素的活性。游离原生质体染色法,对HMC毒素活性的测定,虽同样准确可靠,但其费用较高。相较而言,根冠细胞染色法对玉米小斑病菌的抗性鉴定是一种简便、快捷、实用的检测方法,且能快速筛选出抗病体,为加速抗病育种提供资源基础。
The toxin activity of Helminthosporium maydis race C （HMC） was detected by 3 different methods,and their detection effects were compared.The results showed that isolated leaf acupuncture inoculation could be relatively simple to detect the activity of toxins and lesion formation,but was not carried out for qualitative analysis.The method of root cap cells staining could be used for qualitative as well as qualitative analysis.Although free protoplasts staining method for the determination of HMC toxin activity was accurate and reliable,it cost a lot.In contrast,the method of root cap cell staining is a simple,quick,accurate,economical and applicable method for the determination of disease resistance and can quickly screen the resistant individuals,which provide a material basis for accelerating breeding.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第6期775-779,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
河北省自然科学基金项目(C2011301010)
河北省高层次人才“三三三人才工程”项目(A2010003028)
河北省科技计划项目(13396401D)
农业部“948”项目[2011-G1(3)-07]
科技部国际合作项目(2006DFB02480)
关键词
玉米小斑病菌C小种
毒素
活性
检测
Helminthosporium maydis race C （HMC）
toxin
activity
detection
