摘要
目的:观察不同时间机械通气对慢性阻塞性肺疾病(COPD)大鼠肺脏氧化应激反应的影响。方法:40只雄性Wistar大鼠,随机分为5组:无机械通气正常(N)组、模型(M)组以及机械通气1 h(M1)组、2 h(M2)组、4 h(M3)组,每组8只。熏香烟联合气管内滴注脂多糖(LPS)方法复制大鼠COPD模型。N组和M组直接处死,M1、M2、M3组分别常规潮气量机械通气1、2、4 h后处死。取各组肺组织测定湿/干重(W/D)比值及MDA含量;免疫组化方法检测GCLM蛋白表达情况。结果:与N组相比,M、M1、M2、M3组的W/D和MDA显著增加,M1组高于M组,M2组高于M1组,M3组高于M2组(P<0.05);与N组相比,M1、M2、M3组的GCLM蛋白的表达显著增加,M组小于M1组,M2组大于M1、M3组(P<0.05),M1组与M2组比无明显差异(P>0.05)。结论:机械通气2 h内,COPD大鼠的肺脏水肿程度和氧化应激反应增加不显著,2 h后显著增加,而抗氧化应激反应在2 h内增加,2 h后减弱。
Objective To investigate the effect of mechanical ventilation at different periods on lung oxidative stress in rats with COPD. Methods Forty Wister male rats were randomly divided into 5 groups： normal control group （group N ）, COPD group （group M）, and ventilation groups （groups M1, M2, and M3） based on a series of random number and sample size, 8 for each group, mechanical ventilation was performed according to the following： 1 h in group M1, 2 h in group M2, 4 h in group M3 and no mechanical ventilation in group M. The samples of pulmonary tissue were collected. Tile wet/dry ratio （W/D） and MDA were measured. Immunohistoehemistry was used to detect the expression of GCLM protein. Results The W/D ratio and the MDA content in the lung tissues were higher in groups M, M1, M2, and M3 than in group N （P 〈 0.05）. The W/D ratio and MDA content were higher in groups M2 and M3 than in group M1, and were higher in group M3 than in group M2 （P 〈 0.05）. Levels of GCLM protein in the lung tissues were higher in groups M, M1, M2, and M3 than in group N.Lcvels of GCLM protein were higher in groups M1, M2, and M3 than in group M, and were higher in group M2 than in groups M1 and M3 （P〈0.05） ; there was no significant difference between group M1 and group M3 （P 〉 0.05）. Conclusions Pulmonary edema level and oxidative stress do not increase significantly in rats with COPD but elevate after that. Anti-oxidative stress response increase within 2 h hours and declined after that.
出处
《实用医学杂志》
CAS
北大核心
2013年第22期3640-3642,共3页
The Journal of Practical Medicine
基金
河南省卫生科技创新人才项目(编号:4088)
河南省医学科技重大攻关项目(编号:200902019)
关键词
肺疾病
慢性阻塞性
潮气量
机械通气
氧化应激
Pulmonary disease, chronic obstructive
Tidal volume
Volume ventilator
Oxidative stress
