摘要
蛋白质组学技术提供了从蛋白质整体活动的角度来研究生命活动规律的手段,为研究细胞信号转导提供了新的方法和思路。应用于蛋白质分离鉴定中的双向电泳和液质联用等技术有效地促进了细胞信号转导研究中蛋白之间相互作用,分子伴侣功能的确定,信号通路的关联机制及细胞内信号转导的动态变化等方面的研究。综述近年来蛋白质组学技术在细胞信号转导研究中的应用情况及所取得的成就,并展望未来的发展前景。
Proteomics allows for the exploration of the proteome by looking at the intracellular protein interaction and its own unique activity pattern. This opens the door to studying the cellular signal transduction with new tools and techniques. The application of these new methods, such as two-dimensional gel electrophoresis and liquid chromatography-mass spectrometry, as used in proteome quantification and identification, have promoted the study of the cellular signal transduction effectively. This is seen more so in the research of protein interaction, molecular chaperone functioning, present passway crosstalk, and the dynamic activities of cellular signal transduction. This review presents proteomic applications and a number of the outstanding achievements accomplished in cellular signal transduction research as well as a prediction of the prospective application of these findings in the future.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第11期46-50,共5页
Biotechnology Bulletin
基金
国家自然科学基金项目(31160469)
内蒙古自然基金项目(2011MS0521)
关键词
蛋白质组学
细胞信号转导
串话
Proteomic Cellular signal transduction Cross-talking
