摘要
目的观察新城疫病毒(NDV)对CCl4诱导的小鼠肝纤维化的抑制作用。方法昆明小鼠腹腔注射CCl4/花生油溶液,每周2次,连续8周,制作肝纤维化模型。最后一次CCl4注射后3d由尾静脉注射NDV 1次或3次,每次注射间隔24h,注射完毕24h后处死动物。取出肝脏进行大体形态观察,行HE和天狼猩红染色观察肝组织病理学改变,Western blotting检测肝组织中α-平滑肌肌动蛋白(α-SMA)的表达。结果 CCl4诱导8周后,小鼠肝脏出现明显纤维化表现,可见肝脏质硬、表面粗糙不平、大量密集分布的白色点状斑块。HE染色显示纤维化肝脏组织结构松散,窦周隙增大。天狼猩红染色显示胶原异常沉积。而NDV注射3次后,小鼠肝脏表面白色斑点显著减少,胶原沉积降低。Western blotting结果表明,α-SMA表达水平随NDV注射次数增加而降低。结论 NDV能有效抑制CCl4诱导的小鼠肝纤维化的发生发展。
Objective To explore the inhibitory effect of Newcastle diseases virus （NDV） on CCl4-induced liver fibrosis in mice. Methods Liver fibrosis model was reproduced in 30 Kunming mice by intraperitoneal injection of CCl4/peanut oil solution for 2 times a week, and the total treatment lasted for 8 weeks. Three days after last injection, NDV was injected through tail vein for 1 or 3 times （24h intervals）. Twenty-four hours after NDV infusion, mice were sacrificed and the livers were removed for gross morphology observation. The liver tissue sections were stained by HE and Sirius red dyeing, α-smooth muscle actin （α-SMA） expression was detected by Western blotting. Results After CCl4 induction for 8 weeks, obvious fibrosis symptoms appeared in the liver of model mice, and the surface of liver tissue became hard with rough, with white patches on it. HE staining showed that there was loosening of tissue and enlarged perisinusoidal spaces in liver with fibrosis. Sirius red dyeing displayed abnormal collagen deposition in the fibrotic liver tissues. After NDV injection for 3 times, white spots on the surface of mouse liver were significantly reduced, and collagen deposition was lowered. Western blotting showed that α-SMA levels decreased with increasing frequency of NDV injection. Conclusion NDV may effectively suppress the development of CCl4-induced liver fibrosis in mice.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2013年第11期885-887,共3页
Medical Journal of Chinese People's Liberation Army
基金
陕西省教育厅资助项目(009JK427)
渭南师范学院基金资助项目(10YKZ054)~~
关键词
新城疫病毒
肝硬化
实验性
四氯化碳
newcastle disease virus
liver cirrhosis, experimental
carbon tetrachloride
