摘要
目的:研究生长因子结合受体7(growth factor receptor bound 7,GRB7)在早孕小鼠子宫内膜中的表达规律,探讨其在胚胎着床过程中的作用。方法:分别应用实时荧光定量PCR、免疫组化和Western blot方法检测未妊娠及妊娠早期d1～7小鼠子宫内膜GRB7基因及蛋白的表达;孕3 d下午8点子宫角注射GRB7反义寡核苷酸,孕7 d观察胚泡着床数较正常组有无变化。结果:实时荧光定量PCR测得未妊娠(d0)和妊娠早期(d1～7)小鼠子宫内膜组织均有GRB7 mRNA表达,并在孕5 d达到峰值;免疫组化及Western blot分析显示GRB7蛋白在子宫内膜的表达规律与实时荧光定量PCR基本一致,孕1 d到孕4 d,GRB7蛋白主要表达部位为腔上皮、腺上皮,基质细胞少量表达,孕5 d、孕6 d,GRB7蛋白表达较之前增强,并且在基质细胞开始明显表达,孕5 d最强;孕6 d着床点GRB7蛋白高表达于初级蜕膜区,着床旁GRB7蛋白高表达于基质细胞;子宫角注射GRB7反义寡核苷酸后胚泡着床数较正常组明显减少。结论:GRB7在早孕小鼠子宫内膜中呈动态表达,提示它参与了胚胎着床的发育过程。
Objective：To explore the role of growth factor receptor bound 7 （GRB7） during embryo implantation through detecting its expression pattern in the mouse endometrium. Methods：Expression of GRB7 mRNA and protein in the non-pregnant and pregnant mouse endometrium from dl to d7 was examined by qRT-PCR,immunohistochemistry and Western blot. GRB7 antisense oligo-nu- cleotide was injected at 8 pm on pregnancy d3 and embryo implantation number on pregnancy d7 was examined. Results：GRB7 mR- NA expressed in uterine endometrium of the non-pregnant and pregnant mouse from dl to d7 and reached the highest level on preg- nant d5 based on the results of qRT-PCR. Immunohistochemistry and Western blot showed that the expression pattern of GRB7 in mouse endometrium was the same with that detected by qRT-PCR. Moreover,from pregnant dl to d4,GRB7 was mainly expressed in epithelium and glandular cells and was lowly expressed in stromal cells;on d5 and d6, GRB7 was more intense in epithelium and glandular cells and was obviously expressed in stromal cells, especially on d5. On d6, GRB7 was expressed strongly in primary decid- ual zones at implantation sites and stromal cells at inter-implantation sites, respectively. After horn injecting GRB7 antisense oligonu- cleotides, the number of embryo implantation was obviously decreased when compared with that in control group. Conclusions ： Differ- ential expression of GRB7 in pregnant mouse endometrium suggests that GRB7 may involve in the process of embryo implantation.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2013年第10期1149-1153,共5页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目(编号:31271545)
关键词
生长因子结合受体7
子宫内膜
胚胎着床
]growth factor receptor bound 7
endometrium
embryo implantation
