摘要
目的 :用抗 - HEV ORF2单克隆抗体和抗 - HEV ORF2单链可变区抗体研究急性戊型肝炎的免疫病理学 ,探讨二者在临床病理诊断中的应用价值。 方法 :(1)以杂交瘤技术制备的抗 - HEV单克隆抗体 (Mc Ab,m ono-clonal antibody)作为第一抗体 ,用免疫组化 L SAB法检测急性戊型肝炎患者肝组织中的 HEV抗原。 (2 )用抗 - HEVORF2单链可变区抗体以间接酶标法检测肝组织内的 HEV抗原。 结果 :用抗 - HEV单克隆抗体从 14例急性戊型肝炎肝标本中检出 9例 HEV抗原阳性者 (6 4.2 8% ) ,其中 8例阳性标本用抗 - HEV ORF2单链可变区抗体检测HEVAg均为阳性。HEV抗原在肝细胞浆表达以胞浆弥漫型为主 ,尚可见膜型表达。HEV抗原阳性细胞集中分布在病变明显区 ,伴淋巴细胞浸润 ,病变较轻区也可见随机散在分布 ,部分阳性肝细胞无变性。胆管上皮细胞中见有HEV抗原阳性颗粒。 结论 :本研究结果支持急性戊型肝炎的免疫致病机理。 HEV抗原在胆管上皮细胞的检出可能为 HEV从胆管上皮细胞向胆小管排泌提供了一个直观的证据。同时表明两种抗体可作为一种特异而有效的HEV抗原检测试剂。
Objective: To investigate the pathogenesis of acute hepatitis E with anti HEV monoclonal antibody, and anti HEV ORF2 scF2, and evaluate the application of anti HEV McAb in immunohistochemistry. Methods: Using anti HEV ORF2 McAb and scFv as the primary antibodies, to detect HEVAg in liver tisues from patients with acute hepatitis E by means of immunohistochemistry method (LSAB, Labelled Streptavidin Biotin Method). Results: Hepatitis E virus antigen (HEVAg) was identified in liver tissues of 9 out of 14 cases. The HEVAg distributed diffusely in cytoplasma and cytomembrane of hepatocyte, no positive signals were observed in nuclei. The HEVAg positive cells were concentrated in the severe liver damaged areas with inflammatory infiltration, and also, were scattered singly. Besides, HEVAg positive granules were observed in bile epithelial cells. Conclusion: The results indicate that the acute hepatitis E might be mediated by immunopathogenesis and shows an evidence that HEV may be secreted from biliary epithelial cells to bile duct lumen. Both anti HEV ORF2 McAb and anti HEV ORF2 scFv could be specific and effective reagents in detection of HEVAg.
出处
《新疆医科大学学报》
CAS
2000年第4期327-329,共3页
Journal of Xinjiang Medical University
基金
新疆医科大学第一附属医院科研发展基金! ( 99-YFY-0 4)
关键词
戊型肝炎
抗原
单克隆抗体
单链可变区抗体
hepatitis E virus antigen
monoclonal antibody
single chain Fv antibody
immunohistochemistry
