摘要
以3份扁蓿豆(Medicago ruthenica)幼苗为试验材料,研究了不同NaCl浓度对其细胞膜透性和叶绿素荧光参数的影响,以期为选育扁蓿豆耐盐品种和揭示耐盐机制提供理论依据。结果表明:随着NaCl浓度的增加,3份扁蓿豆材料的细胞膜透性、初始荧光(F_0)、非光化学猝灭(qN)逐渐上升;最大荧光(F_m)、PSⅡ最大光化学效率(F_v/F_m)、潜在光化学活性(F_v/F_0)和光化学猝灭(qP)均呈下降趋势,而PSⅡ非循环光合电子传递速率(ETR)和实际光化学量子产量(y)均先上升后下降;说明NaCl胁迫抑制了PSⅡ的功能,使PSⅡ反应中心活性下降。3份扁蓿豆的叶绿素荧光参数随盐胁迫增加变幅不同,用模糊数学的隶属函数法对3份扁蓿豆的耐盐性进行综合评价,MR2的耐盐性最强。
Cell membrane permeability and chlorophyll fluorescence parameters were studied under the dif- ferent NaC1 treatments using three kinds of Medicago ruthenica seedlings in order to provide the theoreti- cal basis of studying the salt tolerance mechanisms of Medicago ruthenica seedlings and breeding. Results showed that the cell membrane permeability, the minimal fluorescence （F0）, and non photochemical quenching coefficient （qN） increased with the increase of NaC1 concentration, whereas the maximum fluo- rescence （Fro）, maximum photochemical efficiency of PS Ⅱ （Fv/Fm）, PS Ⅱactivity （Fv/Fo） and photo- chemical quenching （qP） decreased. Both PS Ⅱ acyclic photosynthetic electron transfer rate （ETR） and ac- tual photochemical quantum yield （Y） were first increased then decreased. These results indicated that NaC1 inhibited PS Ⅱ functionality and decreased PS Ⅱ center activity. The amplitude of chlorophyll fluo- rescence parameter was different with salt stress increasing among three kinds of Medicago ruthenica. The salt tolerance of Medicago ruthenica was comprehensive evaluated using the membership function of fuzzy mathematics. MR2 showed the highest tolerance to salt.
出处
《草地学报》
CAS
CSCD
北大核心
2013年第5期913-920,共8页
Acta Agrestia Sinica
基金
内蒙古自治区科技计划项目"优良牧草新品种土默特扁蓿豆标准化生产示范"
农业部农作物种质资源保护与利用项目"多年生牧草更新复壮与利用"(NB2013-2130135-48)
农业部牧草种质资源保护项目"牧草温带备份库的维护与备份繁殖入库"(201315)资助
关键词
扁蓿豆
盐胁迫
电导率
叶绿素荧光参数
Medicago ruthenica
Sah-stress
Electric conductivity
Chlorophyll fluorescence
