摘要
利用低能离子束介导转基因法于 1993年首次获得成功 ,本研究通过这一技术将抗菌肽ShivaA基因导入水稻恢复系明恢6 3成熟胚中 ,经过诱导愈伤和绿苗分化 ,并再生植株 ,经PCR检测分析证明外源基因已整合到再生植株基因组中。
The antibacterial polypetide Shiva A genes were transfered into mature embryos of restore line Minhui 63 of hybird rice by low energy ion beam and the transgenic plants were obtained.Foreign gene into genomes of transgenic plants was confirmed by PCR assay.
出处
《安徽农业科学》
CAS
2000年第4期408-409,419,共3页
Journal of Anhui Agricultural Sciences
基金
安徽省"九五"科技攻关课题
关键词
水稻育种
低能离子束介导法
抗茵肽ShivaA
明恢63
Rice breeding
Low energy ion beam
Antibaterial polypetide Shiva A gene
Restore line Minhui63 of hybird rice
