摘要
以黄金蒲桃的茎段为外植体，研究不同质量百分比的氯化汞溶液、不同pH、不同季节取材和不同取材部位对外植体污染率和褐变率的影响，以及不同基本培养基，不同取材部位、不同质量浓度植物生长调节剂组合对芽诱导率和丛芽生长的影响。结果表明，氯化汞的最适质量百分比存0．1％～0．5％之间，最适pH为6．0，冬季取材较好，WPM和Anderson培养基对黄金蒲桃的芽诱导影响差异不大，茎段是比较理想的取材部位，培养的最适植物生长调节剂组合为0．5mg／L6一BA＋0．1mg／LNAA，其芽诱导率高达91．3％，每个茎段平均丛生芽数为3．2。
The stem segment was selected as explant to study the effects of different conditions on the tissue culture of Xan- thostemon chrysanthus. The results were as follows, the optimum concentrations of HgC12 solution ranged from 0.1% to 0. 5% ; the optimum pH was 6.0; the optimum season of material collection was winter; the influences of WPM and Anders- son Medium on the bud induction had no difference; stem segment was the ideal explant; the optimum plant growth regula- tor combination was 0.5 mg/L 6-BA ＋ 0.1 mg/L NAA, in which bud induction rate reached 91.3% , and the average number of inducing buds of per stem segment got 3.2.
出处
《江苏林业科技》
2013年第5期10-13,39,共5页
Journal of Jiangsu Forestry Science & Technology
关键词
黄金蒲桃
芽诱导
组织培养
茎段
Xanthostemon chrysanthus
Bud induction
Tissue culture
Stem segment
