摘要
为了解拟南芥和荠菜形态差异与其生长素极性分布的关系,采用荧光定量PCR,以–actin为内参基因,对拟南芥和荠菜的根、茎、叶、花等组织中与生长素极性运输相关的PIN1、PIN3、PIN7基因的表达进行定量分析。结果表明:拟南芥各组织中PIN1的表达量都高于荠菜各组织中PIN1的表达量,拟南芥的茎和花中PIN1的表达量分别比荠菜茎和花中PIN1表达量高3倍和10倍;拟南芥各组织中PIN7的表达量也高于荠菜各组织的PIN7表达量,其叶片中PIN7的表达量比荠菜叶片中PIN7的表达量高10倍以上;荠菜各组织中PIN3的表达都高于拟南芥相应组织中PIN3的表达量,在茎、叶中的表达分别高3倍和10倍以上,这些生长素极性运输蛋白相关基因表达的差异可能是导致2种植物形态差异的直接原因。
In order to understand the morphogenesis difference of Arabidopsis thaliana and Capsella bursa-pastoris and elucidate the difference of auxin distribution, fluorescence quantitative PCR （qPCR） method with the ？–actin gene as internal reference was used to quantify the expression levels of PIN1, PIN3, PIN7 in the roots, stems, leaves, flowers of the two kinds of plants. The results show that the PIN1 expression in all Arabidopsis thaliana organs is higher than they are in the Capsella organs. The expressions of PIN3 in the stem and flower of Arabidopsis thaliana were 3 and 10 times higher than those in the corresponding organs of Capsella bursa-pastoris. PIN7 expression is also higher in Arabidopsis thaliana than it is in the corresponding organs of Capsella with the expression of PIN7 in the leaf of Aradidopsis thaliana more than 10 times higher than those in the leaf of Capsella bursa-pastoris. PIN3 expression is higher in all tissues of Capsella bursa-pastoris than it is in the Arabidopsis thaliana. The expressions of PIN3 in the stem and flower of Arabidopsis thaliana were 3 and 10 times higher than those in the corresponding organs of Capsella bursa-pastoris. The differential expression of auxin polar transport protein related genes may be the direct reason causing the morphology difference in the two plants.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第5期495-499,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省教育厅重点实验室开放项目(S201201)
关键词
拟南芥
荠菜
PIN基因
定量分析
Arabidopsis thaliana
Capsella bursa-pastoris
PIN genes
quantitative analysis
